437620

Lipopolysaccharide

from E. coli J5 mutant, Nitric oxide synthase stimulator, lyophilized

• Synonym(e): Lipopolysaccharide, E. coli J5
• CAS-Nummer: 93572-42-0
• UNSPSC Code: 12352201
• NACRES: NA.77
• Form: lyophilized
• Storage condition: OK to freeze

Eigenschaften

• Produktname: Lipopolysaccharide, E. coli J5, Mutant of E. coli O111: B4. Stimulates the activity of inducible nitric oxide synthase. Induces apoptosis in mouse thymus and swine lymphocytes.
• Quality Segment: 100
• description: RTECS - OJ0895400
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• impurities: <2.5% Protein, 5.0-10.0% 2-keto-3-deoxyoctonate (KDO)
• solubility: water: 1 mg/mL
• anion traces: phosphate (PO₄^3-): 6.0-15.0%
• shipped in: ambient
• storage temp.: 2-8°C

Beschreibung

General description

Mutant of E. coli 0111:B4. Stimulates the activity of inducible nitric oxide synthase. Induces apoptosis in mouse thymus and swine lymphocytes. Highly purified preparation.

Mutant of E. coli O111: B4. Stimulates the activity of inducible nitric oxide synthase. Induces apoptosis in mouse thymus and swine lymphocytes.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Inducible nitric oxide synthase

Product does not compete with ATP.

Reversible: no

Preparation Note

Following reconstitution refrigerate (4°C). Stock solutions are stable for up to 1 year at 4°C.

LPS will form a suspension in H₂O; slight warming and vortexing may be required to achieve complete dispersion in H₂O. Addition of 0.5% triethylamine will also help with dispersion; triethylamine is very basic and can be neutralized with Trix-HCl to avoid hydrolysis of the fatty acid chains.

Other Notes

Hattori, S., et al. 1995. Biochem. Mol. Biol. Int.35, 177.
Mayeux, P.R., et al. 1995. Biochem. Pharmacol.49, 115.
Norimatsu, M., et al. 1995. Infect. Immun.63, 1122.
Feinstein, D.L., et al. 1993. J. Neurochem.60, 1945.
Zhang, Y.H., et al. 1993. Infect. Immun.61, 5044.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)

Sicherheitshinweise

• Piktogramme: GHS06
• Signalwort: Danger
• Gefahrencodes: H300
• Sicherheitshinweise: P264 - P270 - P301 + P310 - P405 - P501
• Hazard Classifications: Acute Tox. 2 Oral
• Lagerklasse: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable