71238-M

pIEx-2 DNA - Novagen

Novagen′s pIEx vectors are designed for cloning and high-level expression of proteins in transiently transfected Spodoptera derived insect cells.

• Synonym(e): InsectDirect^™ System- pIEx-2 DNA
• UNSPSC-Code: 41106611
• NACRES: NA.51

Eigenschaften

• Qualitätsniveau: 100
• Form: liquid
• Hersteller/Markenname: Novagen^®
• Lagerbedingungen: OK to freeze
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

The pIEx vectors are designed for cloning and high-level expression of proteins in transiently transfected Spodoptera-derived insect cells. Transcription is driven by the AcNPV-derived hr5 enhancer and immediate early promoter, IE1. pIEx-2 contains N-terminal GST•Tag, His•Tag, and S•Tag coding sequences, and a C-terminal HSV•Tag coding sequence. N-terminal vector encoded sequences can be completely removed by cloning into the PshA I site and cleaving the fusion protein with enterokinase.
pIEx-2 is also available as an Ek/LIC vector. The Ek/LIC vector is prepared for rapid directional cloning of PCR-amplified DNA. Using specifically designed primers for amplification, inserts can be efficiently cloned without the need for restriction enzyme digestion or ligation.

For sequencing or PCR of recombinants in pIEx-2, refer to the table below to choose the appropriate primers.

Rechtliche Hinweise

INSECTDIRECT is a trademark of Merck KGaA, Darmstadt, Germany

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable