AB3284

Anti-Endothelin Receptor B Antibody

Chemicon^®, from rabbit

• Synonym(e): ET-B
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IHC, WB
• Citations: 4

Eigenschaften

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat
• manufacturer/tradename: Chemicon^®
• technique(s): immunohistochemistry: suitable, western blot: suitable
• NCBI accession no.: NM_000115.1, NM_003991.1
• UniProt accession no.: P24530
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... EDNRB(1910)

Beschreibung

Immunogen

Purified peptide corresponding to amino acids 298-314 of the rat Endothelin Receptor B (Accession P21451).

Application

Anti-Endothelin Receptor B Antibody detects level of Endothelin Receptor B & has been published & validated for use in IH & WB.

Research Category
Neuroscience

Research Sub Category
CNS Control of Metabolism

Hormones & Receptors

Western blot: 1:200 using ECL on rat brain membranes.

Immunohistochemistry on rat brain sections.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Recognizes Endothelin Receptor B (ET-B). The immunogen sequence is specific for ET-B and is not present in any other known protein.

SPECIES REACTIVITIES: The immunogen sequence is identical in all known vertebrate proteins. Reactivity with other species has not been confirmed.

Physical form

Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

Preparation Note

Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Gefahrencodes: H412
• Sicherheitshinweise: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 3