ABE2595

Anti-H3K4me3Q5amphetaminyl

• Synonym(e): Histone H3 trimethylated Lysine 4 amphetaminylated glutamine 5, Histone H3me3K4Q5apmhetaminyl
• UNSPSC Code: 12352203
• NACRES: NA.46
• Species reactivity: human
• Technique(s): western blot: suitable
• Application: WB
• Citations: -

Eigenschaften

• Quality Segment: 200
• biological source: rabbit
• antibody form: purified antibody
• antibody product type: primary antibodies
• mol wt: calculated mol wt 15.4 kDa, observed mol wt ~16 kDa
• purified by: affinity chromatography
• species reactivity: human
• species reactivity (predicted by homology): rat, mouse
• packaging: antibody small pack of 100
• technique(s): western blot: suitable
• isotype: IgG
• epitope sequence: N-terminal
• Protein ID accession no.: NP_001363866
• UniProt accession no.: P68431
• storage temp.: 2-8°C
• Gene Information: human ... H3C1(8350)

Beschreibung

General description

Histone H3.1 (UniProt: P68431; also known as Histone H3/a, 3/b,3/c, 3/d, 3/e, 3/f, 3/h, 3/i, 3/j, 3/k, 3/l) is encoded by the H3C1 gene (Gene ID: 8350) in human. Histones are highly conserved basic nuclear proteins that are responsible for the nucleosome structure of chromatin in eukaryotes. They play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The amino-terminal tails of histone proteins are subject to posttranslational modifications, including acetylation and methylation, which recruit downstream regulatory factors, influence chromatin structure, and are critical determinants of transcription. Histone H3Q5amphetaminylation is a novel post-translational modification, where the glutamine-5 residue of histone H3 becomes covalently modified by amphetamine (a central nervous system stimulant which is commonly used to treat ADHD and narcolepsy) via transglutaminase 2 (TG2)-mediated incorporation. This modification is structurally similar to histone dopaminylation, which has been implicated in transcriptional regulation and neuronal function. Given the psychoactive properties of amphetamine and it′s long-term use by patients, H3Q5 amphetaminylation could in theory influence gene expression patterns in a drug-specific manner, potentially affecting neuronal plasticity, addiction-related pathways, and other neuronal processes. Amphetaminylation of glutamine 5 on histone H3 is recently identified as a permissive post-translational modification that coexists with adjacent lysine 4 trimethylation.  Characterization of this modification could provide insight into how amphetamines modify long-term epigenetics in the brain and body, with implications for neuropsychiatric disorders, substance use research, and more.

Immunogen

KLH-conjugated linear peptide corresponding to 10 amino acids from the N-terminal region of human Histone H3.1 modified with tri-methylation at lysine 4 and amphetamine at glutamine 5 position.

Application

Quality Control Testing

Evaluated by Western Blotting in Recombinant TG2, Recombinant mononucleosomes reacted with amphetamine.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Histone H3.1 modified with amphetamine at Glutamine 5, in Recombinant TG2 + Recombinant mononucleosomes reacted with 5mM amphetamine.

Biochem/physiol Actions

This rabbit polyclonal antibody specifically detects Histone H3.1 modified with tri-methylation at lysine 4 and amphetamine at glutamine 5 position.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

0.15 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Analysis Note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable