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Unterstützung erhaltenform
tablet
packaging
pkg of 20 tablets (11112422001[50mg]), pkg of 20 tablets (11204521001[5mg])
manufacturer/tradename
Roche
color
green
solubility
water: soluble
λmax
414 nm
shipped in
wet ice
storage temp.
2-8°C
General description
ABTS Tablets are used as substrates in enzyme immunoassays with horseradish peroxidase as a marker enzyme. It is widely used in spectrophotometric methods to measure antioxidant activities of food and beverages.[1]
Application
ABTS Tablets are the ideal substrate for enzyme immunoassays with horseradish peroxidase (HRP) as a marker enzyme.
Features and Benefits
- ABTS is soluble in aqueous and organic solvents
- ABTS method is suitable for various pH levels
- Samples react with ABTS quickly and reach stability within 30 minutes
Packaging
- 11204521001 (5 mg/tablet; each tablet is for 5 mL substrate solution)
- 11112422001(50 mg/tablet; each tablet is for 50 mL substrate solution)
Preparation Note
Working solution: Dissolve one ABTS tablet (5 mg) in 5 ml of the ABTS buffer solution. This solution has a light-green color. In microtiter plates, it is colorless A405 nm/1 cm should be <0.16. Work as clean as possible. Heavy metal ions or traces of peroxidase can produce a dark green ABTS solution. Such solutions are not suitable for the assay.
Analysis Note
Evaluation: photometric (405nm)
Other Notes
For life science research only. Not for use in diagnostic procedures.
Legal Information
ABTS is a trademark of Roche
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1
Lagerklasse
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
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Haralg Seitz and Sarah Schumacher
Molecular Diagnostics null
R Re et al.
Free radical biology & medicine, 26(9-10), 1231-1237 (1999-06-25)
A method for the screening of antioxidant activity is reported as a decolorization assay applicable to both lipophilic and hydrophilic antioxidants, including flavonoids, hydroxycinnamates, carotenoids, and plasma antioxidants. The pre-formed radical monocation of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS*+) is generated by oxidation
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