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97622

2-Mercaptoethanol

derivatization grade (HPLC), LiChropur™, ≥99.0% (GC)

Synonym(e):

β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylenglycol

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Lineare Formel:
HSCH2CH2OH
CAS-Nummer:
Molekulargewicht:
78.13
UNSPSC Code:
23151817
NACRES:
NA.05
PubChem Substance ID:
EC Number:
200-464-6
Beilstein/REAXYS Number:
773648
MDL number:
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grade

derivatization grade (HPLC)

Quality Segment

vapor density

2.69 (vs air)

vapor pressure

1 mmHg ( 20 °C)

assay

≥99.0% (GC)

form

liquid

quality

LiChropur™

expl. lim.

18 %

concentration

14.3 M (pure liquid)

technique(s)

HPLC: suitable, LC/MS: suitable, ion chromatography: suitable

refractive index

n20/D 1.500 (lit.), n20/D 1.500-1.502

bp

157 °C (lit.)

density

1.114 g/mL at 25 °C (lit.)

storage temp.

2-8°C

SMILES string

OCCS

InChI

1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2

InChI key

DGVVWUTYPXICAM-UHFFFAOYSA-N

General description

2-Mercaptoethanol is a thiol compound showing growth promoting properties on mouse lymphoma L1210 cells in vitro. It also enhances viability, blast transformation and antibody formation in lymphocyte cultures.

Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.
suitable for derivatisation of Amine groups

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

LiChropur is a trademark of Merck KGaA, Darmstadt, Germany

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Dieser Artikel
M3148PHR9274444203
description

derivatization grade (HPLC), LiChropur™, ≥99.0% (GC)

description

Molecular Biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

description

certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

description

A disulfide-reducing agent.

technique(s)

HPLC: suitable

technique(s)

electrophoresis: suitable, cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

grade

derivatization grade (HPLC)

grade

Molecular Biology

grade

certified reference material, TraceCERT®

grade

Molecular Biology

assay

≥99.0% (GC)

assay

99% (GC/titration)

assay

-

assay

≥99% (GC)

Quality Level

100

Quality Level

200

Quality Level

300

Quality Level

100

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

15-25°C

form

liquid

form

liquid

form

-

form

liquid


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Signalwort

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral

Zielorgane

Liver,Heart

Lagerklasse

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Flammpunkt (°F)

165.2 °F - closed cup

Flammpunkt (°C)

74 °C - closed cup



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Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

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