E6003

Eosin Y Dinatriumsalz

Dye content ≥85%, Powder

• Synonym(e): 2′,4′,5′,7′-Tetrabromfluorescein Dinatriumsalz, Bromfluorescein, D&C; Rot Nr. 22, Eosin gelblich, Säurerot 87
• Empirische Formel (Hill-System): C₂₀H₆Br₄Na₂O₅
• CAS-Nummer: 17372-87-1
• Molekulargewicht: 691.85
• UNSPSC Code: 12171500
• NACRES: NA.47
• PubChem Substance ID: 24894349
• EC Number: 241-409-6
• MDL number: MFCD00005040
• Colour Index Number: 45380
• Beilstein/REAXYS Number: 3586809

Eigenschaften

• Produktname: Eosin Y Dinatriumsalz, ≥85 % by HPLC
• form: powder
• Quality Segment: 200
• composition: , ≥85% HPLC
• technique(s): titration: suitable
• color: brown
• pH: 6.55 (29 °C, 84 °F)
• mp: 255 — 270 °C (491 - 518 °F at 977.9 hPa)
• solubility: H₂O: 1 mg/mL
• density: 1.018 g/cm³
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].[Na+].[O-]C(=O)c1ccccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c([O-])c(Br)cc24
• InChI: 1S/C20H8Br4O5.2Na/c21-11-5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(22)17(26)15(24)19(10)29-18(9)14(23)16(11)25;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2
• InChI key: SEACYXSIPDVVMV-UHFFFAOYSA-L

Beschreibung

Application

Eosin Y disodium salt has been used for determining sperm viability. The swollen and stained spermatozoa were considered dead. In corneal epithelium, it has been used in the procedure of immunohistological staining.

Biochem/physiol Actions

Eosin Y is a xanthene dye and is used for the differential staining of connective tissue and cytoplasm. In histopathology, it is applied as a counterstain after hematoxylin and before methylene blue. It is also used as a background stain, thereby giving contrast to the nuclear stains.

Sicherheitshinweise

• pictograms: GHS07
• signalword: Warning
• hcodes: H317,H319
• pcodes: P261 - P264 - P272 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Sens. 1
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: 376.9 °F - Pensky-Martens closed cup
• flash_point_c: 191.6 °C - Pensky-Martens closed cup
• ppe: dust mask type N95 (US), Eyeshields, Gloves