Fast Green FCF has long been cited for use in plant histology. This product has not been tested for suitability in plant protein staining, including yogurts. The solubility of this product is tested in water at 1 mg/mL. Various literature indicate higher rates may be achieved. Please see the following link to review a publication that may be helpful:
https://pmc.ncbi.nlm.nih.gov/articles/PMC8834546/
Fortfahren mit
F7258
Fast Green FCF
certified by the BSC
Synonym(e):
Lebensmittelgrün 3
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
25 g | Warenkorb auf Verfügbarkeit prüfen | € 198,00 |
Über diesen Artikel
Lineare Formel:
C37H34N2O10S3Na2
CAS-Nummer:
Molekulargewicht:
808.85
EC Number:
219-091-5
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
5718212
Colour Index Number:
42053
Technischer Dienst
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Unterstützung erhaltenagency
certified by the BSC
Quality Segment
form
powder
composition
dye content, ≥90%
mp
290 °C (dec.) (lit.)
solubility
H2O: 1 mg/mL
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c2ccc(cc2)C(=C3/C=CC(\C=C3)=[N+](\CC)Cc4cccc(c4)S([O-])(=O)=O)\c5ccc(O)cc5S([O-])(=O)=O
InChI
1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2
InChI key
RZSYLLSAWYUBPE-UHFFFAOYSA-L
Application
Fast Green FCF has been used:
Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.
Zertifiziert für die Safranin-Echtgrün FCF-Färbung von Pflanzenzellen; als Ersatz für Anilinblau in der Einstufen-Gomori-Trichrom-Färbung.
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Dieser Artikel | |||
|---|---|---|---|
| description certified by the BSC | description analytical standard | description Dye content ≥85 % | description certified by the BSC |
| solubility H2O: 1 mg/mL | solubility - | solubility H2O: 1 mg/mL, clear | solubility H2O: 1 mg/mL, blue |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level 200 |
| form powder | form - | form powder | form powder |
| storage temp. room temp | storage temp. - | storage temp. - | storage temp. room temp |
| application(s) diagnostic assay manufacturing | application(s) cleaning products | application(s) - | application(s) diagnostic assay manufacturing |
| agency certified by the BSC | agency - | agency - | agency certified by the BSC |
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Signalwort
Warning
Gefahrencodes
Sicherheitshinweise
Hazard Classifications
Muta. 2 - STOT SE 3
Zielorgane
Respiratory system
Lagerklasse
11 - Combustible Solids
WGK
WGK 2
Flammpunkt (°F)
352.4 °F - closed cup
Flammpunkt (°C)
178 °C - closed cup
PSA (Persönliche Schutzausrüstung)
Eyeshields, Gloves
Hier finden Sie alle aktuellen Versionen:
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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
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We're developing wheat/rice flour + pea protein yogurts and want to image them by CLSM. Is this Fast Green FCF suitable for fluorescent protein staining? What concentration and solvent do you recommend?"
1 answer-
Helpful?
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Looking for a green dye to add to electrolyzed water. Something biodegradable that will disappear fast on skin.
1 answer-
It is advised to source a FD&C (Food, Drug & Cosmetic) grade dye for use on human skin. Assuming there is suitable electrolyzed water, green food coloring should suffice for the intended purpose. The duration for which the dye remains on the skin would depend on factors such as the pH of the water used and the specific characteristics of the dye, which the customer would need to determine.
Helpful?
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What is the proper method to prepare Fast Green FCF for use as a counterstain?
1 answer-
Solutions of Fast Green FCF are used much like solutions of Light Green SF Yellowish. The advantage of using Fast Green FCF is generally that the stain is more intense. The working solution is typically prepared fresh each day or used for a short period of time.
Helpful?
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