04529

Nitrilase

recombinant, expressed in E. coli, ≥2.0 U/mg

• Synonym(e): Nitrile aminohydrolase
• CAS-Nummer: 9024-90-2
• UNSPSC Code: 12352204
• NACRES: NA.54
• EG-Nummer: 3.5.5.1 (BRENDA, IUBMB)
• MDL number: MFCD04040969
• Specific activity: ≥2.0 U/mg
• Recombinant: expressed in E. coli

Eigenschaften

• recombinant: expressed in E. coli
• form: crystals, powder
• specific activity: ≥2.0 U/mg
• mol wt: 41 kDa
• storage temp.: 2-8°C

Beschreibung

General description

Nitrilase belongs to the C-N hydrolase superfamily. It possesses the catalytic triad motifs: glutamate, lysine, and cysteine and are distributed in fungi, plants, and animals.The isoelectric point (pI) of this protein is 8.1. Native polyacrylamide gel electrophoresis (PAGE).

Application

Nitrilase may be used for immobilization on acryloyl crosslinked cellulose dialdehyde (ACCD) for catalytic activity studies.

Biochem/physiol Actions

Nitrilases catalyze the liberation of ammonia and the formation of carboxylic acid from the nitriles. The oligomeric structural organization of nitrilase is crucial for its functionality. It displays broad substrate specificity.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

1 U corresponds to the amount of enzyme which liberates 1 μmol ammonia per minute at pH 7.2 and 25°C with the conversion of acrylonitrile to acrylic acid

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• Gefahrencodes: H317,H334
• Sicherheitshinweise: P261 - P280 - P342 + P311
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable