DAPI is soluble in water (10 mg/mL) with heat or sonication as necessary. It is insoluble in PBS even at 1 mg/ml.
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32670
4′,6-Diamidin-2-phenylindol -dihydrochlorid
suitable for fluorescence, BioReagent, ≥95.0% (HPLC)
Synonym(e):
2-(4-Amidinophenyl)-6-indolcarbamidin -dihydrochlorid, DAPI -dihydrochlorid
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
5 mg | Warenkorb auf Verfügbarkeit prüfen | € 92,90 |
25 mg | Warenkorb auf Verfügbarkeit prüfen | € 326,00 |
Über diesen Artikel
Empirische Formel (Hill-System):
C16H15N5 · 2HCl
CAS-Nummer:
Molekulargewicht:
350.25
UNSPSC Code:
12171500
NACRES:
NA.32
PubChem Substance ID:
EC Number:
249-186-7
Beilstein/REAXYS Number:
4894417
MDL number:
Technischer Dienst
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Unterstützung erhaltenproduct line
BioReagent
Quality Segment
assay
≥95.0% (HPLC)
form
powder
solubility
DMF: soluble, H2O: soluble
fluorescence
λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex), λex 374 nm; λem 461 nm in 10 mM Tris; 1 mM EDTA; pH 8.0; DNA
suitability
suitable for fluorescence
storage temp.
room temp
SMILES string
Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
InChI
1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
InChI key
FPNZBYLXNYPRLR-UHFFFAOYSA-N
Application
DAPI ist um ein Vielfaches empfindlicher als Ethidiumbromid zur DNA-Färbung in Agarosegelen. Es kann für sogenanntes Photo-Footprinting von DNA verwendet werden, zum Nachweis gebundener Sonden in Blotting-Anwendungen durch gezielte Visualisierung des doppelsträngigen Komplexes, als auch um Änderungen in der DNA zu untersuchen und um den DNA-Gehalt während der Apoptose mittels Durchflusszytometrie zu bestimmen. DAPI-Färbung ist eine sensitive und spezifische Nachweismethode für Mykoplasmen.
DAPI ist um ein Vielfaches sensitiver als Ethidiumbromid für die Färbung von DNA in Agarosegelen.
Biochem/physiol Actions
Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
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Dieser Artikel | |||
|---|---|---|---|
| description suitable for fluorescence, BioReagent, ≥95.0% (HPLC) | description Cell-permeable DNA-binding dye. | description For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS)., 1 mg/mL | description For Nuclear counterstain in Fluorescence Microscopy |
| suitability suitable for fluorescence | suitability - | suitability suitable for nucleic acid staining | suitability suitable for nucleic acid staining |
| fluorescence λex 364 nm; λem 454 nm (DAPI-DNA-Komplex), λex 374 nm; λem 461 nm in 10 mM Tris; 1 mM EDTA; pH 8.0; DNA, λex 340 nm; λem 488 nm (nur DAPI) | fluorescence λem ~461 nm, λex ~359 nm | fluorescence λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex) | fluorescence λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex) |
| solubility DMF: soluble, H2O: soluble | solubility water: 5 mg/mL, ethanol: soluble, methanol: soluble | solubility water: soluble | solubility - |
| form powder | form solid | form liquid | form liquid |
| assay ≥95.0% (HPLC) | assay ≥90% (HPLC) | assay - | assay - |
| storage temp. room temp | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
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Signalwort
Warning
Gefahrencodes
Sicherheitshinweise
Hazard Classifications
Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3
Zielorgane
Respiratory system
Lagerklasse
11 - Combustible Solids
Was ist los?
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
PSA (Persönliche Schutzausrüstung)
Eyeshields, Gloves, type N95 (US)
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How can I make a solution of 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), Product 32670?
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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What concentration of 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), Product 32670, is used to as a nuclear stain for cultured cells?
1 answer-
Use DAPI at 0.1 microgram per mL in phosphate buffered saline (PBS) to incubate with monolayer cultured cells for 15-30 minutes at 30°C. Immediately examine with a fluorescence microscope.
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How can I store solutions of 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), Product 32670?
1 answer-
At 1 mg/mL, the solutions are stable in the dark at 2-8 °C for several weeks. For long-term storage, the solutions can be divided into aliquots and stored frozen at -20°C
Helpful?
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What concentration of 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), Product 32670, is used to as a nuclear counterstain for tissue sections?
1 answer-
Following fluorescent staining of the cells, DAPI is added to the mounting media at 2 μg/ml.
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