46067

Ethidiumbromid -Lösung

suitable for fluorescence, ~1% in H₂O

• Synonym(e): 3,8-Diamino-5-ethyl-6-phenyl-phenanthridiniumbromid, EtBr, Homidiumbromid
• Empirische Formel (Hill-System): C₂₁H₂₀BrN₃
• CAS-Nummer: 1239-45-8
• Molekulargewicht: 394.31
• UNSPSC Code: 12161505
• NACRES: NA.32
• PubChem Substance ID: 57650684
• MDL number: MFCD00011724
• Beilstein/REAXYS Number: 3642536

Eigenschaften

• Quality Segment: 100
• concentration: ~1% in H₂O
• fluorescence: λ_ex 480 nm; λ_em 620 nm in H₂O, λ_ex 518 nm; λ_em 605 nm (bound to DNA), λ_ex 530 nm; λ_em 600 nm in 50 mM phosphate buffer pH 7.0 (upon binding to DNA)
• suitability: suitable for fluorescence
• storage temp.: 2-8°C
• SMILES string: [Br-].CC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14
• InChI: 1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H
• InChI key: ZMMJGEGLRURXTF-UHFFFAOYSA-N

Beschreibung

Application

Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.

Biochem/physiol Actions

Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.

Preparation Note

Für die Gelfärbung nach der Elektrophorese Stammlösung mit Wasser zu 0.5 μg/ml verdünnen und Gel für 15-30 min. inkubieren. Entfärben ist normalerweise nicht notwendig, kann aber gegebenenfalls für 15 min in Wasser durchgeführt werden um den Hintergrund zu veringern. Die DNA-Banden können dann in einer UV light box (Wellenlänge 254 nm) sichtbar gemacht werden. Es besteht auch die Option Ethidiumbromid in Gel und Laufpuffer zuzugeben (0.5 μg/ml), was eine Visualisierung unmittelbar nach der Elektrophorese ermöglicht.

Sicherheitshinweise

• pictograms: GHS06,GHS08
• signalword: Danger
• hcodes: H331,H341
• pcodes: P202 - P261 - P271 - P280 - P304 + P340 + P311 - P308 + P313
• Hazard Classifications: Acute Tox. 3 Inhalation - Muta. 2
• Lagerklasse: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter