This product has not been tested for labeling bacteria internally, including Staphylococcus aureus. Therefore, no data is available for this application. A literature search is recommended for specific applications.
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46950
Fluoreszein-5(6)-isothiocyanat
BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)
Synonym(e):
FITC
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
50 mg | Warenkorb auf Verfügbarkeit prüfen | € 39,60 |
250 mg | Warenkorb auf Verfügbarkeit prüfen | € 114,00 |
1 g | Warenkorb auf Verfügbarkeit prüfen | € 346,00 |
Über diesen Artikel
Empirische Formel (Hill-System):
C21H11NO5S
CAS-Nummer:
Molekulargewicht:
389.38
UNSPSC Code:
12171500
NACRES:
NA.32
PubChem Substance ID:
EC Number:
248-207-7
Beilstein/REAXYS Number:
1407295
MDL number:
Technischer Dienst
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Unterstützung erhaltenQuality Segment
product line
BioReagent
assay
≥90% (HPLC)
form
powder
mol wt
389.38 g/mol
color
orange to dark orange, yellow to dark yellow
solubility
acetone: 0.1 g/10 mL, clear to very slightly hazy
fluorescence
λex 492 nm; λem 518 nm in 0.1 M phosphate pH 8.0
suitability
suitable for fluorescence
storage temp.
−20°C
SMILES string
Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
InChI
1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
InChI key
MHMNJMPURVTYEJ-UHFFFAOYSA-N
General description
Fluorescein 5(6)-isothiocyanate, also known as FITC, is a weakly acidic hydroxyxanthene whose overall size is small, with both carboxylic acid and isothiocyanate substituents on the pendant phenyl ring. The dye has a moderately sized, nonplanar aromatic system. The isothiocyanate group reacts with nucleophilic substituents (e.g. amino, hydroxyl, thiol) of biomolecules to be attached as a fluorescent label. The maximum emission of the dye is at 519nm in the same solvent.
Application
Für die Fluoreszenzmarkierung von Proteinen (z.B. Immunofluoreszenz); H Protein-Labeling für die Gelchromatographie.
Fluorescein 5(6)-isothiocyanate (FITC) labelled molecules are used in non-microscopic immunoassays and physiological tracing experiments. FITC is widely used in flow cytometry experiments for staining proteins, nuclei, and subnuclear particles. White blood cells (WBCs) are marked with FITC to study their response toward a chemoattractant, eventually being measured.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
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Dieser Artikel | |||
|---|---|---|---|
| description BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC) | description suitable for protein labeling, ≥90% (HPLC), powder | description ≥97.5% (HPLC) | description suitable for fluorescence, ≥90% (HPLC), BioReagent |
| suitability suitable for fluorescence | suitability suitable for protein labeling | suitability - | suitability suitable for fluorescence, corresponds for coupling to thiols |
| fluorescence λex 492 nm; λem 518 nm in 0.1 M phosphate pH 8.0 | fluorescence λex 492 nm; λem 518 nm (green) | fluorescence λex 492 nm; λem 518 nm (green) | fluorescence λex 490 nm; λem 518 nm in 0.1 M Tris pH 8.0 |
| color yellow to dark yellow, orange to dark orange | color orange to dark orange | color yellow to very dark yellow | color - |
| solubility acetone: 0.1 g/10 mL, clear to very slightly hazy | solubility acetone: 1 mg/mL | solubility acetone: soluble 10 mg/mL | solubility - |
| form powder | form powder | form powder | form - |
| assay ≥90% (HPLC) | assay ≥90% (HPLC) | assay ≥97.5% (HPLC) | assay ≥90% (HPLC) |
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Lagerklasse
11 - Combustible Solids
PSA (Persönliche Schutzausrüstung)
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Signalwort
Warning
Gefahrencodes
Sicherheitshinweise
Hazard Classifications
Acute Tox. 4 Oral - Skin Irrit. 2 - Skin Sens. 1B
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excuse me， can the product of Fluorescein 5(6)-isothiocyanate be used to lable bacteria，such as Staphylococcus aureus？ thanks
1 answer-
Helpful?
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Is it suitable for fluorescence microscopy?
1 answer-
This product is tested for fluorescent labeling of proteins. While not tested specifically for use in fluorescent microscopy, this compound is routinely used in cell-based studies applying this technique.
Please see the link below to review the Peer Reviewed Publications:
https://www.sigmaaldrich.com/search/46950?focus=papers&page=1&perpage=30&sort=relevance&term=46950&type=citation_searchHelpful?
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