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Lipase aus Rhizopus oryzae

Name: Lipase aus <I>Rhizopus oryzae</I>
Brand: SIGMA

powder (fine), ~10 U/mg

Synonym(e):

Lipase aus Rhizopus arrhizus, Triacylglycerol-acylhydrolase, Triacylglycerol-lipase

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Über diesen Artikel

CAS-Nummer:

9001-62-1

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-619-9

EG-Nummer:

3.1.1.3 (BRENDA, IUBMB)

MDL number:

MFCD00131509

Specific activity:

~10 U/mg

Technischer Dienst

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Unterstützung erhalten

Eigenschaften

form

powder (fine)

Quality Segment

100

specific activity

~10 U/mg

mol wt

M_r ~43000

storage temp.

2-8°C

InChI

1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;

InChI key

QWZUIMCIEOCSJF-CHHCPSLASA-N

Beschreibung

General description

Research Area: Cell Signaling
Rhizopus oryzae lipase (ROL) is a protein synthesized in a precursor form that includes a presequence of 26 amino acids, followed by a prosequence of 97 amino acids, which is attached to the N-terminal of a mature sequence consisting of 269 amino acids.

Lipase from Rhizopus oryzae (ROL) comprises an oxyanion hole, four N-glycosylation sites, and an active site region. It possesses N-terminal presequence and prosequence.

Application

Lipase from Rhizopus oryzae has been used:

to test its effect on 1,2-diolein synthesis and triolein ethanolysis
for immobilization on graphene oxide support for biocatalysis studies
to digest triglycerides (TAG) from Chlamydomonas reinhardtii and S. cerevisiae

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Biochem/physiol Actions

Lipase from Rhizopus oryzae (ROL) acts as a catalyst for the enzymatic biosynthesis of polyglycerol polyricinoleate through a reversal of hydrolysis. ROL is useful in the industrial production of structured lipids due to its 1,3-regiospecificity functionality.

Rhizopus oryzae lipase (ROL) has been extensively researched for its regiospecificity in biodiesel production. Due to its remarkable characteristics, including 1,3-specificity, high enantioselectivity, and stability in organic solvents, ROL has garnered significant attention for applications in the energy, food, and pharmaceutical industries.

Tri-, Di-, und Monoglyceride sind hydrolysiert (in abnehmender Ratenreihenfolge).

Other Notes

1 U entspricht der Enzymmenge, die 1 μmol Buttersäure pro Minute bei pH 8.0 und 40°C freisetzt (Tributyrin, Fluka^®-Nr. 91010 als Substrat) 5000 U wie oben beschrieben entsprechen ~1 U, wenn Triolein (Fluka Nr. 62314) als Substrat, bei pH 8.0 und 40°C, verwendet wird

Catalyst for the interesterification of oils and fats; For removal of interfering triglycerides in the electroimmunoassay of apolipoprotein B; Racemic epoxy ester resolution through enantioselective enzymatic hydrolysis.

Note: When triacetin is used as substrate, the pH is 7.4. Incubation time: 60 minutes.