This product can be used for SDS-PAGE in-gel detection of His-tagged proteins; however, imaging after Coomassie staining with CBB G350 is not the preferred workflow. For maximum fluorescence signal, image the gel before Coomassie staining.
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94159
NTA – Atto 550
BioReagent, suitable for fluorescence, ≥80% (sum of isomers, HPCE)
Synonym(e):
Nα,Nα-bis-(carboxymethyl)-L-lysin, Nickel(II)-Komplex, Atto 550 - Nitrilotriessigsäure, komplexiert mit Nickel
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
250 μg | Warenkorb auf Verfügbarkeit prüfen | € 213,00 |
Über diesen Artikel
NACRES:
NA.32
UNSPSC Code:
41116100
Technischer Dienst
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Unterstützung erhaltenproduct line
BioReagent
Quality Segment
assay
≥80% (sum of isomers, HPCE)
form
lumps
fluorescence
λex 554 nm; λem 576 nm in PBS with 0.08% sodium azide
suitability
suitable for fluorescence
detection method
fluorometric
storage temp.
−20°C
Application
für die Fluoreszenzdetektion von His-markierten Proteinen
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Dieser Artikel | |||
|---|---|---|---|
| description BioReagent, suitable for fluorescence, ≥80% (sum of isomers, HPCE) | description BioReagent, suitable for fluorescence, ≥80% (sum of isomers, HPCE) | description BioReagent, suitable for fluorescence, ≥80% (HPCE) | description suitable for fluorescence, ≥90% (HPLC) |
| suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence |
| fluorescence λex 554 nm; λem 576 nm in PBS with 0.08% sodium azide | fluorescence λex 501 nm; λem 523 nm in PBS with 0.08% sodium azide | fluorescence λex 647 nm; λem 669 nm in PBS | fluorescence - |
| form lumps | form solid | form - | form powder |
| assay ≥80% (sum of isomers, HPCE) | assay ≥80% (sum of isomers, HPCE) | assay ≥80% (HPCE) | assay ≥90% (HPLC) |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
Signalwort
Danger
Gefahrencodes
Sicherheitshinweise
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1A - Eye Irrit. 2 - Muta. 2 - Repr. 1A - Resp. Sens. 1 - Skin Sens. 1 - STOT RE 1 Inhalation
Lagerklasse
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Was ist los?
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
PSA (Persönliche Schutzausrüstung)
dust mask type N95 (US), Eyeshields, Gloves
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Can NTA-ATTO 550 be used for in SDS-PAGE gel detection after staining with CBB G350?
1 answer-
Helpful?
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What is the recommended stock solution concentration and usage concentration of NTA-ATTO 550?
1 answer-
This product is soluble in water at 20 µL per vial. The usage concentration is dependent on specific application details. An application discussion can be found on our website link:
https://www.sigmaaldrich.com/technical-documents/technical-article/protein-biology/protein-labeling-and-modification/ni-nta-atto-conjugatesHelpful?
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what is the molecular weight of NTA - Atto 550?
1 answer-
Unfortunately, the molecular weight of this product is not determined.
Helpful?
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What are the best conditions to store the reconstituted fluorophore? What would the shelf-life be under such conditions?
1 answer-
Upon receipt the material can be stored between 0 to -20 °C. After reconsitituion, the material can be
stored at 4 °C. The stability of the product has not been determined in reconstituted format.Helpful?
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