To ensure stability until the recommended retest date printed on the lot specific certificate of analysis, A1031 should be maintained at -20C after receipt. Transport conditions may differ from the recommended long-term storage conditions.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/232/426/storage-transport-conditions-ms.pdf
Fortfahren mit
A1031
α-Amylase aus Humanspeichel
Type XIII-A, lyophilized powder, 300-1,500 units/mg protein
Nachhaltigkeitsmerkmale
Synonym(e):
1,4-α-D-Glucan-glucanohydrolase
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
1000 units | Warenkorb auf Verfügbarkeit prüfen | € 123,00 |
5000 units | Warenkorb auf Verfügbarkeit prüfen | € 449,00 |
Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-565-6
NACRES:
NA.54
MDL number:
EG-Nummer:
Specific activity:
300-1,500 units/mg protein
Technischer Dienst
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Unterstützung erhaltentype
Type XIII-A
Quality Segment
form
lyophilized powder
specific activity
300-1,500 units/mg protein
composition
Protein, 5-15% biuret
sustainability
Sustainability features
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... AMY1A(276)
General description
Humane α-Amylase besteht aus 496 Aminosäuren in einer einzelnen Polypeptidkette, die auf Chromosom 1 kodiert ist. Es wird entweder in der Schilddrüse oder der Pankreas produziert. Die Speichel- und Pankreas-α-Amylasen haben eine homologe Primärsequenz, weisen aber unterschiedliche Spaltungsmuster auf.
Wir sind darauf bedacht, Ihnen umweltfreundlichere Alternativprodukte anzubieten, die mit einem oder mehreren der „12 Prinzipien der Grünen Chemie“ in Einklang stehen. Dieses Produkt wurde für den Einsatz in der Stärke/Ethanol-Forschung im Hinblick auf Energieeffizienz und Abfallvermeidung verbessert. Weitere Informationen sind im Biofiles-Artikel angegeben.
Application
α-Amylase wird zur Hydrolyse von α-Bindungen α-verknüpfter Polysaccharide wie Stärke und Glykogen verwendet. Produkt A1031 stammt aus menschlichem Speichel, ist vom Typ IIA und wird als lyophilisiertes Pulver geliefert. α-Amylase wird in verschiedenen Pflanzenstudien wie beispielsweise zur Untersuchung des Stoffwechsels in Arabidopsis eingesetzt. α-Amylase aus menschlichem Speichel wird zur Untersuchung der Entwicklung von Nutrazeutika verwendet, die in der Behandlung von Diabetes und Adipositas hilfreich sein können .
Biochem/physiol Actions
Physical form
Lyophilisiertes Pulver mit (NH4)2SO4, Natriumcitrat und Natriumchlorid.
Other Notes
Eine Einheit setzt in 3 min bei pH 6,9 und 20 °C 1,0 mg Maltose aus Stärke frei.
Für weitere Informationen siehe enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
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Dieser Artikel | |||
|---|---|---|---|
| description Type XIII-A, lyophilized powder, 300-1,500 units/mg protein | description Type IX-A, lyophilized powder, 1,000-3,000 units/mg protein | description fractionated antiserum, lyophilized powder | description recombinant, expressed in HEK 293 cells |
| specific activity 300-1,500 units/mg protein | specific activity 1,000-3,000 units/mg protein | specific activity - | specific activity - |
| Gene Information human ... AMY1A(276) | Gene Information human ... AMY1A(276) | Gene Information human ... AMY1A(276) | Gene Information human ... AMY1A(276) |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| UniProt accession no. | UniProt accession no. | UniProt accession no. - | UniProt accession no. |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
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Signalwort
Danger
Gefahrencodes
Sicherheitshinweise
Hazard Classifications
Resp. Sens. 1
Lagerklasse
11 - Combustible Solids
Was ist los?
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Hier finden Sie alle aktuellen Versionen:
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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
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Can this be stored at room temperature until it is reconstituted or does it always need to be kept at -20C?
1 answer-
Helpful?
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Quanti mg di enzima sono contenuti in una confezione?
1 answer-
This product is sold by units and vial content will vary from lot to lot. The minimum activity of this enzyme is 300 units per milligram. The following values indicate the least amounts that will be present in each pack size:
1KU~ 3.3mg
5KU~16.6 mgThe exact specific activity will be listed on the lot specific Certificate of Analysis. Please see the link below to view a Certificate of Analysis: https://www.sigmaaldrich.com/product/sigma/a1031#product-documentation
Helpful?
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Considering the wide range of activity range, how can I be sure of the enzyme activity of the specific batch that I receive?
1 answer-
The protein content and enzymatic activity are determined on a lot to lot basis. Each value is reported in the specific Certificate of Analysis. The protein content range is 5 - 15% and the enzymatic activity range is 300 - 1500 units per milligram protein. A review of recent lots indicates typical protein concentrations of 7 - 13% and activities of 1350 - 1450 units per milligram protein. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/a1031#product-documentationHelpful?
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¿Cómo se reconstituye la enzima al venir liofilizada? ¿Qué tasa de actividad tiene?
1 answer-
For assay purposes, this enzyme is reconstituted in water at 1 u/mL. This material is also soluble in 1 mM Calcium Chloride. The activity specification is 300 - 1500 U/mg. The unit per milligram value is reported in the lot-specific Certificate of Analysis and will range from 0.67 to 3.3 mg for the 1000 U pack size. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/a1031#product-documentationHelpful?
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Hi, could you let me know what the remaining composition is when the product contains 5-15% protein? Thank you!
1 answer-
As mentioned in the 'DESCRIPTION' section, this product is a lyophilized powder containing (NH4)2SO4, sodium citrate and sodium chloride. This product is not tested for purity. However, buffer salts are expected to be the bulk of the non-protein content.
Helpful?
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Please let me know the storage temperature and storage period when it is dissolved in water
1 answer-
The stability of this material in solution has not been investigated. However, various sources suggest that solutions may be stored in working aliquots at -20°C for several months.
Helpful?
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What is the recommended method for dissolving alpha amylase obtained from human saliva, specifically A1031?
1 answer-
The recommended method is to dissolve the enzyme in 1mM calcium chloride (CaCl2).
Helpful?
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