At high concentrations, Alginate lyase may degrade the entire extracellular matrix too rapidly and damage the structural integrity of encapsulated cells or tissue constructs. It may also cause unwanted aggregation or precipitation of breakdown products, and the high protein concentration itself might interfere with downstream applications.
Fortfahren mit
A1603
Alginat-Lyase
powder, ≥10,000 units/g solid
Synonym(e):
poly-beta-D-mannuronate lyase
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
100 mg | Warenkorb auf Verfügbarkeit prüfen | € 267,00 |
Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
12352204
NACRES:
NA.54
EG-Nummer:
MDL number:
Specific activity:
≥10,000 units/g solid
Technischer Dienst
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Unterstützung erhaltenGeneral description
Alginat-Lyasen kommen in mehreren Meeresalgen, in Meeresweichtieren und in einer Vielzahl von marinen und terrestrischen Bakterien vor.
Application
Alginat-Lyase wird zum Abbau von Alginat oder Alginsäure und zur Verringerung der Viskosität verwendet. Es ist bei der Follikelisolierung, Einkapselung und Kultivierung von Nutzen .
Alginat-Lyase wird verwendet:
- bei der Vorverarbeitung des Enzyms
- zur Entkapselung von Zellen
- für die Verdauung des Pseudomonas--Exopolysaccharids zur Erleichterung der Elektroporation
Biochem/physiol Actions
Spaltet Alginat oder Alginsäure in kleinere Moleküle auf und verringert die Viskosität.
Alginat-Lyasen beteiligen sich aktiv an der Verzuckerung der sauren Polysaccharide für die Produktion von Bioethanol.
Preparation Note
0,15 mL Enzymlösung (1 un/mL) wird 4,5 mL 0,1%igem Natriumalginat (pH 6,3) zugegeben. Bei 37 °C 30 Minuten lang inkubieren. Reaktion durch Zugabe von 4,65 mL 0,1 N NaOH beenden.
Other Notes
Eine Einheit erzeugt einen Anstieg von A235 nm von 1,0 pro Minute pro mL Natriumalginatlösung bei pH 6,3 und 37 °C
Weitere Informationen über Enzyme für die Analyse komplexer Kohlenhydrate finden Sie unter www.sigma-aldrich.com/enzymeexplorer
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Dieser Artikel | |||
|---|---|---|---|
| description powder, ≥10,000 units/g solid | description lyophilized powder, ≥0.20 unit/mg solid | description 20% aldehyde content, medium viscosity | description 35% aldehyde content, medium viscosity |
| specific activity ≥10,000 units/g solid | specific activity ≥0.20 unit/mg solid | specific activity - | specific activity - |
| form powder | form lyophilized powder | form powder | form powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
Signalwort
Danger
Gefahrencodes
Sicherheitshinweise
Hazard Classifications
Resp. Sens. 1
Lagerklasse
11 - Combustible Solids
Was ist los?
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
PSA (Persönliche Schutzausrüstung)
Eyeshields, Gloves, type N95 (US)
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I read on your site, that Alginat lyase should be resuspended at a concentration of 0.05 - 1.5 u/ml in 0.1 mol/L Phosphate buffer (pH 6.3). What if I prepared 24 U/ml (1mg contains 24U). Can this be harmful for the sample?
1 answer-
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Hi, is this enzyme a G-lyase (specific for GG-linkages) or M-lyase (specific for MM-linkages)?
1 answer-
This enzyme degrades GG linkages approximately twice as effectively as MM linkages.
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Hi! What is the PL family, source and structure of this enzyme?
1 answer-
Most information on this enzyme is proprietary. However, it is an endo-type enzyme sourced from Flavobacterium species and functions as an endo-cleaving enzyme.
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What are the aminoacid sequence of the enzyme, molecular weight, molar extinction and the original bacterium
1 answer-
The amino acid sequence and molar extinction of this enzyme has not been determined. The molecular weight is approximately 40,000 daltons. The specific species of the bacterium is considered proprietary.
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Hi, could you please tell me the source of the alginate lyase and the strain in which it was expressed? Thank you!
1 answer-
This enzyme is produced by microbial fermentation. The specific species is considered proprietary.
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I was wondering how to prepare the enzyme solution and what does the 1 un/ml mean? Is is 1 unit/mL or what does the un stand for? How do I convert the units to grams?
1 answer-
A1603 should be resuspended at a concentration of 0.05 - 1.5 u/ml in 0.1 mol/L Phosphate buffer (pH 6.3).
The specific activity of each lot (units/gram solid) is provided on the certificate of analysis. The lot specific certificate of analysis can be found here:
https://www.sigmaaldrich.com/product/sigma/a1603#product-documentation
Based on the specific activity (units/gram solid), the researcher can weigh out the appropriate amount of A1603 to achieve a 1 unit/mL solution.
un = unitHelpful?
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Hi, I've ordered this product from here, but it's just saying powder, ≥10,000 units/g solid, can you please tell me the molecular weight of it? in Da or kDa?
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The molecular weight this material is approximately 40,000 daltons.
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I ordered the alginate lyase from your company. But the information that I received only present >=10,000U/g. And I need the 20U/m in my experiment. Could you give me more information?
1 answer-
The exact specific activity value is specified on the lot specific Certificate of Analysis. Please see this example Certificate of Analysis: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/A1603-BULKSLCQ2940.pdf.
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