A4108

Aminophenyl-Fluorescein-Lösung

5 mM in DMF, suitable for fluorescence

• Synonym(e): 2-[6-(4′-Amino)-phenoxy-3H-xanthen-3-on-9-yl]-benzoesäure-Lösung, APF
• UNSPSC Code: 12352108
• EC Number: 200-679-5
• NACRES: NA.32

Eigenschaften

• form: liquid
• Quality Segment: 200
• concentration: 5 mM in DMF
• suitability: suitable for fluorescence
• storage temp.: 2-8°C

Beschreibung

General description

Aminophenyl fluorescein solution, also known as APF, is a novel fluorescent probe. APF exists as a non-fluorescent entity, which converts to its fluorescent form called fluorescein upon oxidation. The excitation length is at 488nm, and the emission spectrum is monitored between 495-600nm and measured using flow cytometry.

Fluorescence intensity increases after reaction with hROS (e.g. hydroxy radical, peroxynitrite and hypochlorite). And, APF is resistant to light-induced autoxidation.

Application

Aminophenyl fluorescein solution is a suitable tool to detect hypobromous acid (HOBr) produced in eosinophils. It is used to detect the halogenating activity of heme peroxidases in leukocytes. APF does not react with nitric oxide, superoxide, and hydrogen peroxide; hence, it can be used to detect peroxynitrite differentially.

Fluoreszenzdetektor hoch reaktiver Sauerstoffspezies (HROS). Die Fluoreszenzintensität nimmt nach der Reaktion mit HROS (z.B. Hydroxyradikal, Peroxynitrit und Hypochlorit) zu. Da APF nicht mit Stickstoffmonoxid, Superoxid und Wasserstoffperoxid reagiert, kann es verwendet werden, um differentiell Peroxynitrit nachzuweisen. APF ist beständig gegen Licht-induzierte Autoxidation.

Sicherheitshinweise

• Piktogramme: GHS02,GHS08,GHS07
• Signalwort: Danger
• Gefahrencodes: H226,H312 + H332,H319,H360D
• Sicherheitshinweise: P210 - P280 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Eye Irrit. 2 - Flam. Liq. 3 - Repr. 1B
• Lagerklasse: 3 - Flammable liquids
• Was ist los?: WGK 2
• Flammpunkt (°F): 135.5 °F - closed cup
• Flammpunkt (°C): 57.5 °C - closed cup
• PSA (Persönliche Schutzausrüstung): Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter