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A5175

Anti-Human Lambda Light Chains (Bound and Free)−Peroxidase antibody produced in goat

Name: Anti-Human Lambda Light Chains (Bound and Free)−Peroxidase antibody produced in goat
Brand: SIGMA

affinity isolated antibody, buffered aqueous solution

Synonym(e):

Goat Anti-Human Lambda Light Chains (Bound and Free)-HRP

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UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162530

Conjugate:

peroxidase conjugate

Clone:

polyclonal

Application:

DB, ELISA (d), IHC (p)

Citations:

Technischer Dienst

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Unterstützung erhalten

Eigenschaften

biological source

goat

Quality Segment

200

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:35,000, dot blot: 1:40,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Beschreibung

General description

Mammalian antibodies contain one of two types of light chain, called kappa or lamba. Each chain contains a constant and a variable domain. Mammalian antibodies contain either two kappa or two lambda light chains.
Horseradish Peroxidase (HRP) is an enzyme that catalyzes the conversion of chromogenic substrates such as o-phenylenediamine (OPD), 4-chloro-1-naphthol 3,3′,5,5′-tetramethylbenzidine (TMB), 3,3′-Diaminobenzidine (DAB) or 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS); chemiluminescent substrates such as CPS-3 (enhanced luminal) and fluorogenic substrates such as Ampliflu^™ Red into detectable chromophores, light-emitters or fluorescers, respectively.

Application

Anti-Human Lambda Light Chains (Bound and Free)?Peroxidase antibody produced in goat has been used in:

enzyme linked immunosorbent assay (ELISA)
immunohistology
dot blot
detecting and quantitating human lambda light chains (bound and free) via chromogenic, chemoluminescent or fluorogenic immunochemical or immunohistochemical techniques

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)
Western Blotting (1 paper)

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Legal Information

Ampliflu is a trademark of Sigma-Aldrich Co. LLC

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.