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A6667

Anti-Rabbit IgG (whole molecule), F(ab′)2 fragment−Peroxidase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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Größe/SKUVerfügbarkeitPreis
1 mL
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€ 278,00

Über diesen Artikel

UNSPSC Code:
12352203
NACRES:
NA.54
MDL number:
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
ELISA (d)
Citations:
80

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biological source

goat

Quality Segment

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:10,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

IgG antibodies regulate several functions such as complement activation and phagocytosis. Thus they play a crucial role in facilitating cytological immune responses. Anti-rabbit IgG (whole molecule), F(ab′)2 fragment-peroxidase antibody can be used to detect immune-complexes. The product can also be used in direct ELISA. Goat anti- rabbit IgG (whole molecule), F(ab′)2 fragment-peroxidase antibody reacts specifically with all rabbit immunoglobulins.

Immunogen

Purified rabbit IgG.

Application

Anti-rabbit IgG (whole molecule), F(ab′)2 fragment-peroxidase antibody can be used in immunoprecipitation and western blotting.
Proteins from lysed Leishmania amazonensis cells were analyzed by western blot using HRP-conjugated goat anti-rabbit Fc specific IgG as the secondary antibody.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with preservative.

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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affinity isolated antibody, buffered aqueous solution

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technique(s)

direct ELISA: 1:10,000

technique(s)

direct ELISA: 1:10,000

technique(s)

direct ELISA: 1:10,000

technique(s)

direct ELISA: 1:7,000

biological source

goat

biological source

goat

biological source

rabbit

biological source

goat

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

shipped in

dry ice

shipped in

-

shipped in

dry ice

shipped in

wet ice


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Warning

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Lagerklasse

12 - Non Combustible Liquids

wgk

WGK 3



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Questions

  1. Hi, my lab recently purchased this product. I just noticed that the specification doesn't show the recommended concentration for immunoblots. As we purchased it for doing westerns, we were wondering if this product is at all compatible. Thank you.

    1 answer
    1. This antibody is NOT tested for western blot application. There are references that site using this antibody at dilutions of 1:200- 1:500.

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