A7653

L-Alanin-Dehydrogenase aus Bacillus subtilis

buffered aqueous glycerol solution, ~30 units/mg protein (Lowry)

• Synonym(e): L-Alanin: NAD⁺ -Oxidoreduktase
• CAS-Nummer: 9029-06-5
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-847-9
• MDL number: MFCD00131441
• EG-Nummer: 1.4.1.1 (BRENDA, IUBMB)
• Specific activity: ~30 units/mg protein (Lowry)
• Biological source: Bacillus subtilis

Eigenschaften

• biological source: Bacillus subtilis
• Quality Segment: 200
• form: buffered aqueous glycerol solution
• specific activity: ~30 units/mg protein (Lowry)
• foreign activity: LDH ~1% (using pyruvate as substrate)
• storage temp.: −20°C

Beschreibung

Application

L-Alanine dehydrogenase converts L-alanine to pyruvate and ammonium. L-Alanine dehydrogenase from Bacillus subtilis may be used to study enzyme inactivation and protection .

Biochem/physiol Actions

L-Alanine dehydrogenase is an A-stereospecific dehydrogenase that catalyzes the reversible deamination of L-alanine to pyruvate and ammonium. It is important for the generation of pyruvate during sporulation. L-Alanine dehydrogenase from Bacillus subtilis has a predominately ordered kinetic mechanism in which NAD binds before L-alanine. Subsequently, ammonia, pyruvate, and NADH are released in that specific order. Optimal pH for the amination reaction is 8.8-9.0, whereas it is 10-10.5 for the deamination reaction. The enzyme is inactivated by divalent metal ions and p-chloromercuribenzoate, mercuric ion being most effective. The inactivation may be reversed by L- or D-cysteine.

Physical form

Solution in 50% glycerol containing 10 mM potassium phosphate buffer, pH 7.7

Other Notes

One unit will convert 1.0 μmole of L-alanine to pyruvate and NH₃ per min at pH 10.0 at 25 °C.

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• Gefahrencodes: H334
• Sicherheitshinweise: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 10 - Combustible liquids
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)