Chicken IgG refers to antibodies from the chicken serum and chicken IgY antibodies are from the yolk.
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A9046
Anti-Chicken IgY (IgG) (whole molecule)−Peroxidase antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Synonym(e):
Anti Chicken Igy
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
1 mL | Warenkorb auf Verfügbarkeit prüfen | € 227,00 |
Über diesen Artikel
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
DB, ELISA (d), IHC (p)
Citations:
95
Technischer Dienst
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Unterstützung erhaltenbiological source
rabbit
Quality Segment
conjugate
peroxidase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
chicken
technique(s)
direct ELISA: 1:30,000, dot blot: 1:160,000 (indirect chemiluminescence), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Chicken IgY is the major antibody found in chicken serum. Chickens generate a comparatively enhanced antibody response to mammalian antigenic proteins. These immunoglobulins do not bind to Fc receptors present in mammals and bacteria, and also do not interact with rheumatoid factors. Thus anti-chicken antibodies are useful analytical tools for various research applications.
Rabbit Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody binds to chicken IgG.
Rabbit Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody binds to chicken IgG.
Immunogen
purified chicken IgG
Application
Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody produced in rabbit has been used in indirect enzyme-linked immunosorbent assay (ELISA), dierct ELISA, dot blot and immunohistochemistry.
Proteins isolated from rabbit glomeruli by iron oxide magnetization were subjected to western blot analysis using HRP-conjugated rabbit anti-chicken IgY as the secondary antibody.
Rabbit Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody has been used for immunoprecipitation and western blot (1:2,000) assays. The antibody can also be used for direct ELISA (1:30,000), dot blot (1:12,000-1:15,000) and IHC (1:1,000) applications.
Biochem/physiol Actions
Egg yolk IgY immunoglobulin acquired from hen have been studied extensively. It is useful in immunological assays and does interact with the rheumatoid factor.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT
Preparation Note
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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| description affinity isolated antibody, buffered aqueous solution | description IgG fraction of antiserum, buffered aqueous solution | description affinity isolated antibody, buffered aqueous solution | description affinity isolated antibody, buffered aqueous solution |
| technique(s) dot blot: 1:160,000 (indirect chemiluminescence), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000, direct ELISA: 1:30,000 | technique(s) direct ELISA: 1:30,000, dot blot: 1:160,000 using indirect chemiluminescence system using 5 ng human IgG/dot and chicken anti-human IgG as the primary antibody and Luminol plus enhancer as the substrate., immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using using human pancreas sections and chicken anti-human insulin as the primary antibody. | technique(s) indirect ELISA: 1:25,000 | technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40, western blot (chemiluminescent): suitable, dot blot: 1:100,000, direct ELISA: 1:35,000 |
| species reactivity chicken | species reactivity chicken | species reactivity chicken | species reactivity - |
| biological source rabbit | biological source rabbit | biological source rabbit | biological source rabbit |
| antibody form affinity isolated antibody | antibody form IgG fraction of antiserum | antibody form affinity isolated antibody | antibody form affinity isolated antibody |
| conjugate peroxidase conjugate | conjugate peroxidase conjugate | conjugate unconjugated | conjugate alkaline phosphatase conjugate |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
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Warning
Gefahrencodes
Sicherheitshinweise
Hazard Classifications
Skin Sens. 1
Lagerklasse
12 - Non Combustible Liquids
WGK
WGK 2
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How is chicken IgY different from chicken IgG?
1 answer-
Helpful?
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Do we have additional anti-chicken IgY conjugates?
1 answer-
We provide anti-chicken IgY antibodies that are conjugated to alkaline phosphatase (Product No. A9171) and FITC (Product No. F8888). We also provide the unconjugated anti-chicken IgY antibody (Product No. C2288).
Helpful?
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Will Product A9046, Anti-Chicken IgY, also recognize chicken IgG?
1 answer-
This antibody (and possibly other anti-chicken antibodies) recognizes both chicken IgG from serum and chicken IgY from egg yolk, because these immunoglobulins are structurally very similar.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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Product A9046, Anti-Chicken IgY, is peroxidase conjugated. Is this similar to horseradish peroxidase?
1 answer-
The peroxidase used to make this product is indeed peroxidase from horseradish (HRP).
Helpful?
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