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B1522

Basal Medium Eagle

Name: Basal Medium Eagle
Brand: SIGMA

With Earle′s salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(e):

BME

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Über diesen Artikel

UNSPSC Code:

12352207

NACRES:

NA.75

Preise und Verfügbarkeit sind derzeit nicht verfügbar.

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Eigenschaften

Produktname

Basal Medium Eagle, With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Segment

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

L-glutamine: no
NaHCO₃: yes
Earle’s salts (5% CO₂): yes

shipped in

ambient

storage temp.

2-8°C

Beschreibung

General description

Eagle′s Basal Medium (BME) is one of the most used synthetic cell culture media. The tissue culture association recommends that the term "Eagle′s Basal Medium" be used only to refer to the formula developed to support HeLa cells. BME has previously been used in monolayer culture studies to assess the growth response of normal (WI-38) and transformed (mouse and HeLa) cells. BME is the precursor of Dulbecco′s Modified Eagle′s medium (DME) and Eagle′s Minimum Essential Medium (MEM).

Application

Basal Medium Eagle has been used:

as a component in the culture medium for cochlear organotypic cultures and salicylate treatment
to maintain cerebellar granule neurons (CGNs) to determine if there is a system that regulates the distribution and/or group dynamics of axonal mitochondria
in the preparation of cortical slices, serum-free medium, and slice culture medium for slice overlay assay to study the influence of extracellular signals on neuronal development

Preparation Note

Supplement with 0.292 g/L L-glutamine.

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Dieser Artikel	M0268	M4642	M2279
SAFC B1522 Basal Medium Eagle	Sigma-Aldrich M0268 Minimum Essential Medium Eagle	Sigma-Aldrich M4642 Minimum Essential Medium Eagle	Sigma-Aldrich M2279 Minimum Essential Medium Eagle
description With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture	description With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture	description With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture	description With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
form liquid	form powder	form powder	form liquid
sterility sterile-filtered	sterility -	sterility -	sterility sterile-filtered
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
components L-glutamine: no NaHCO₃: yes Earle’s salts (5% CO₂): yes	components HEPES: no phenol red: yes Earle’s salts (5% CO₂): yes NaHCO₃: no L-glutamine: yes sodium pyruvate: no	components glucose: yes Hanks’ salts (2% CO₂): yes L-glutamine: yes Earle’s salts (5% CO₂): no NaHCO₃: no phenol red: yes sodium pyruvate: no stable glutamine: no HEPES: no	components phenol red: yes sodium pyruvate: no L-glutamine: no HEPES: no NaHCO₃: yes Earle’s salts (5% CO₂): yes
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C

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Lagerklasse

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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