C0278

Chlorperoxidase aus Caldariomyces fumago

buffered aqueous suspension, ≥3,000 units/mL

• Synonym(e): Chlorid-Peroxidase
• CAS-Nummer: 9055-20-3
• UNSPSC Code: 12352204
• NACRES: NA.54
• EG-Nummer: 1.11.1.10 (BRENDA, IUBMB)
• MDL number: MFCD00130774
• Concentration: ≥3,000 units/mL

Eigenschaften

• form: buffered aqueous suspension
• Quality Segment: 200
• mol wt: 42 kDa
• concentration: ≥3,000 units/mL
• shipped in: wet ice
• storage temp.: 2-8°C

Beschreibung

General description

Chloroperoxidase is a heme containing glycoprotein that is secreted from fungus. Chloroperoxidase (CPO) is a extracellular heme glycoenzyme containing ferriprotoporphyrin IX as the prosthetic group.

Application

It has been used to study biocatalytic oxidation in polymersome nanoreactors.

Chloroperoxidase from Caldariomyces fumagois is useful alternative to lactoperoxidase for ¹³¹I ion labeling studies, for bromination of proteins, and for ³⁶Cl labeling of macromolecules in long-term isolation procedures. It has been used to study biocatalytic oxidation in polymersome nanoreactors .

A useful alternative to lactoperoxidase for ¹³¹I ion labeling studies, for bromination of proteins, and for ³⁶Cl labeling of macromolecules in long-term isolation procedures.

Biochem/physiol Actions

Chloroperoxidase (CPO) is secreted from fungus and exhibits a broad spectrum of chemical reactivities. It is a peroxide-dependent chlorinating enzyme and it also catalyzes peroxidase, catalase and cytochrome P450-type reactions of dehydrogenation, hydrogenperoxide (H₂O₂) decomposition and oxygen insertion, respectively. The enzyme has magnetic and spectroscopic properties similar to that of cyctochrome P-450. CPO from the fungus Caldariomyces fumago has the capacity to chlorinate aromatic hydrocarbons, including polycyclic aromatic hydrocarbons (PAHs). PAHs are considered to be a potential health risk because of their possible carcinogenic and mutagenic activities and are widely dispersed in the environment.

Physical form

Crude suspension in 0.1 M sodium phosphate, pH ~4.5

Other Notes

One unit will catalyze the conversion of 1.0 μmole of monochlorodimedon to dichlorodimedon per min at pH 2.75 at 25 °C in the presence of potassium chloride and H₂O₂.

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• Gefahrencodes: H334
• Sicherheitshinweise: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 10 - Combustible liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)