Fortfahren mit
C1622
Cyclohexanon-Monooxygenase aus Acinetobacter sp.
≥12 U/mL, ammonium sulfate suspension, recombinant, expressed in E. coli
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
1 mL | Warenkorb auf Verfügbarkeit prüfen | € 120,00 |
Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
12352204
NACRES:
NA.54
EG-Nummer:
MDL number:
Recombinant:
expressed in E. coli
Concentration:
≥12 U/mL
€ 120,00
Warenkorb auf Verfügbarkeit prüfen
Technischer Dienst
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Unterstützung erhaltenrecombinant
expressed in E. coli
Quality Segment
form
ammonium sulfate suspension
concentration
≥12 U/mL
storage temp.
−20°C
Application
Cyclohexanone monooxygenase has been used in a study that cloned and overexpressed the 2-oxo-Δ(3)-4,5,5-trimethylcyclopentenylacetyl-CoA monooxygenase (OTEMO) in Escherichia coli. Cyclohexanone monooxygenase has also been used in a study that investigated the effects of structural modification of the cell wall on the biotransformation capability by recombinant Corynebacterium glutamicum cells.
Biochem/physiol Actions
Purified cyclohexanone monooxygenase has a molecular weight of 59,000 Da. It is a versatile oxygenation catalyst. The enzyme uses the bound FAD-4a-OOH oxygenating intermediate to initiate transfer of oxygen to electrophilic substrate sites. The reaction consequently yields the corresponding sulfoxide and selenoxide products. This enzyme is also capable of oxygenating at nitrogen, trivalent phosphorus, and boron sites in boronic acids. Hence, it is one of the most broad-based flavoprotein oxygenases known.
Physical form
Suspension in 80% saturated ammonium sulfate, 20 mM K-Na-phosphate buffer pH 7, 3.5 mM 1,4-Dithioerythritol (DTE)
Other Notes
1 unit corresponds to the amount of enzyme which catalyzes the cyclohexanone-stimulated oxidation of 1 μmol of NADPH per minute at pH 9.0 and 30 °C
1 of 1
Dieser Artikel | |
|---|---|
| description ≥12 U/mL, ammonium sulfate suspension, recombinant, expressed in E. coli | description recombinant, expressed in E. coli ≥12 units/mg protein |
| form ammonium sulfate suspension | form powder |
| concentration ≥12 U/mL | concentration - |
| recombinant expressed in E. coli | recombinant expressed in E. coli ≥12 units/mg protein |
| storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 |
Signalwort
Danger
Gefahrencodes
Sicherheitshinweise
Hazard Classifications
Resp. Sens. 1
Lagerklasse
11 - Combustible Solids
Was ist los?
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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