Trypsin is still the most widely employed enzyme used for cell detachment. PromoCell´s Trypsin Solutions exhibit standardized enzyme activity and contain trypsin from extra-pure lots. This Trypsin/EDTA Solution has a ratio of 0.04% trypsin and 0.03% EDTA and is available in different sizes.
Store at -20°C in the dark immediately after arrival. Refrigerate thawed solution at 4 to 8°C. If stored properly, the product is stable until the expiry date stated on the label.
Analysis Note
All lots of PromoCell Cell Detachment Reagents are subjected to comprehensive quality control tests. Each lot is routinely tested for biological function, absence of cytotoxicity, and physical parameters such as osmolality and pH level. Approved in-house lots are used as a reference. In addition, all lots have been tested for the absence of microbial contaminants (fungi, bacteria).
1 ×, sterile; sterile-filtered, BioReagent, suitable for cell culture, 0.5 g porcine trypsin and 0.2 g EDTA, 4Na per liter of Hanks′ Balanced Salt Solution with phenol red
technique(s)
cell culture | mammalian: suitable
technique(s)
cell culture | mammalian: suitable
technique(s)
cell culture | mammalian: suitable
technique(s)
single cell analysis: suitable, cell culture | mammalian: suitable