C4187

Cyanoborhydrid-Kupplungspuffer

• Synonym(e): Coupling buffer
• NACRES: NA.56
• UNSPSC Code: 12161700

Eigenschaften

• form: liquid
• Quality Segment: 200
• reaction suitability: reagent type: reductant
• technique(s): affinity chromatography: suitable
• application(s): life science and biopharma
• storage temp.: 2-8°C

Beschreibung

Application

Ein gebrauchsfertiges Supplement für die Kopplung von Amin-Liganden an Aldehydgruppen. Die Reaktion des Kupplungspuffers ist eine reduktive Aminierung der Schiffs-Basen-Zwischenstufe zu einer stabilen C−N-Bindung.

Cyanoborohydride Coupling Buffer is used in affinity chromatography, protein chromatography, activated/functionalized matrices and synthetic reagents. Cyanoborohydride has been used to inform a safe and effective gene-transfer system targeting hepatocytes as well as to develop a method for targeted delivery of anticancer therapeutics to cancer cells in hypoxic areas.

Cyanoborohydride Coupling Buffer has been used:

• in coupling reactions between amines and glutaraldehyde
• to reduce hydrazone bond to a stable hydrazide bond
• as a component in oligonucleotide reaction mixture for coverslips functionalization

Biochem/physiol Actions

Cyanoborohydride Coupling Buffer is a reagent suitable for reductive amination processes, that contributes to transformation of simple alcohols into more complex amines. It is used in the conversion of Schiff base, by reducing it, to form a secondary amine without affecting aldehyde groups on the support.

Preparation Note

0.02 M sodium phosphate, pH 7.5, containing 0.2 M sodium chloride and 3.0 g/L sodium cyanoborohydride

Sicherheitshinweise

• Piktogramme: GHS07,GHS09
• Signalwort: Warning
• Lagerklasse: 12 - Non Combustible Liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Gefahrencodes: H302 + H312,H411
• Sicherheitshinweise: P264 - P273 - P280 - P301 + P312 - P302 + P352 + P312 - P362 + P364
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 2