C8554

Choriongonadotropin

lyophilized powder, suitable for mouse embryo cell culture

• Synonym(e): Choriogonin, HCG
• CAS-Nummer: 9002-61-3
• UNSPSC Code: 12352202
• EC Number: 232-660-2
• NACRES: NA.32
• MDL number: MFCD00130797
• Biological source: human urine
• Form: lyophilized powder

Eigenschaften

• biological source: human urine
• Quality Segment: 200
• sterility: aseptically processed
• form: lyophilized powder
• potency: ≥5,000 I.U. per mg
• mol wt: 37.9 kDa, apparent mol wt 37.9 kDa by SDS-PAGE
• drug control: Home Office Schedule 4.2
• technique(s): cell culture | embryo: suitable
• impurities: HBV,HCV and HIV, tested negative
• solubility: H₂O: 1 mL/vial, saline: 1 mL/vial
• UniProt accession no.: P01215
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... CGA(1081)

Beschreibung

General description

Das Glykoproteinhormon, das aus einer α-Kette mit 92 Aminosäuren besteht, die der von LH, FSH und TSH entspricht; und einer eindeutigen β-Kette mit 149 Aminosäuren. Wird produziert vom Chorionzottengewebe und ist verantwortlich für die Aufrechterhaltung des Gelbkörpers während der frühen Schwangerschaft. Wird auch von Throphoblastzellenneoplasmen produziert, die sich durch erhabene, freie hCG-β-Ketten und hyperglykosyliertem hCG auszeichnen.

Note: Actual molecular weight will vary from the theoretical depending upon the degree of glycosylation. Theoretical MW is 37.9 kDa based on native form which contains 2 subunits: α = 14.9 kDa (~10.2 kDa for the polypeptide and ~4.7 kDa for the carbohydrate), and β = 23.0 kDa (~16.0 kDa for the polypeptide and ~7.0 kDa for the carbohydrate). See J. Biol. Chem. 248, 6796-6827 (1973).

Application

Recommended for superovulation induction in mice.

Biochem/physiol Actions

Human chorionic gondadotropin (hCG) may be used as an alternative to PMSG to induce ovulation in laboratory animals. When hCG was injected into laboratory animals via intravenous (i.v.), intraperitoneal (i.p.), or subcutaneous (s.c.) methodstet, the effective dose values for 50% of the animals (ED50) per animal and per body weight (kg) in parenthesis were as follows:
Mice: i.v. 0.2 (7.7) I.U.; i.p. 0.3 (11.5) I.U.; s.c. 0.7 (26.9) I.U.
Syrian hamsters: i.v. 1.0 (9.5) I.U.; i.p. 1.8 (17.1) I.U.; s.c. 2.6 (24.8) I.U.
Rats: i.v. 1.3 (4.6) I.U.; i.p. 3.5 (12.3) I.U.; s.c.7.5 (26.3) I.U.

Using PMSG, the ED50 values per animal and per body weight (kg) in parenthesis were as follows:
Mice: i.v. 0.8 (30.8) I.U.; i.p. 2.0 (76.9) I.U.; s.c. 2.8 (107.7) I.U
Syrian hamsters: i.v., 3.6 (34.3) I.U.; i. p., 8.0 (76.2) I.U.; s.c., 13.2 (125.7) I.U.
Rats: i.v., 6.0 (76.8) I.U.; i p., 20.8 (73.0) I.U.; s.c., 76.8 (269.5) I.U.

Sicherheitshinweise

• pictograms: GHS08
• signalword: Warning
• hcodes: H361
• pcodes: P281
• Hazard Classifications: Repr. 2
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable