LIM1899 cell line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 12062001, human colon, Epithelial; cells are small rounded and grow in clusters

• Synonym(e): LIM 1899, LIM-1899
• UNSPSC Code: 41106514
• Biological source: human colon
• Growth mode: adherent
• Morphology: Epithelial; cells are small rounded and grow in clusters
• Manufacturer/tradename: ECACC

Eigenschaften

• biological source: human colon
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 12062001-DNA-5UG, pkg of vial of cells 12062001-1VL
• growth mode: adherent
• morphology: Epithelial; cells are small rounded and grow in clusters
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

Biochem/physiol Actions

Columnar cell adenocarcinoma of the colon. Cell Bank Australia reference number: CBA0163.

Preparation Note

Optimal split ratio 1:8 (seeding density 1 x 10⁴ cells/cm²). Cells incubated at 37°C with 5% CO₂. Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min. LIM1899 requires the growth medium to be changed 3 times each week.

RPMI-1640 (with 2mM L-Glutamine + 25mM HEPES) + 10% FCS, Insulin 0.6 μg/ml, Hydrocortisone 1 μg/ml, 1-Thioglycerol 10 μM

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments.  Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.