HeLa Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93021013, human cervix, Epithelial

• Synonym(e): HeLa Cells, HeLa-CCL2 Cells, Henrietta Lacks Cells
• UNSPSC Code: 41106514
• Biological source: human cervix
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: aneuploidy
• Morphology: Epithelial
• Manufacturer/tradename: ECACC

Eigenschaften

• biological source: human cervix
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 93021013-DNA-5UG, pkg of vial of cells 93021013-1VL
• growth mode: Adherent
• karyotype: aneuploidy
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

Application

Virus studies

HeLa cell line has been used to study whether Zika virus (ZIKV) infection alters centrosome number and spindle positioning in neural progenitor cells. It has also been used to evaluate the cytotoxicity of extracts, fruits and jams from Sorbus sps.

Biochem/physiol Actions

Derived from a cervical carcinoma from a 31 year old female. This was the first aneuploid line derived from human tissue maintained in continuous cell culture. Susceptible to Poliovirus type I and adenovirus type 3. Identified as a contaminant in many other cell lines. The cells should be handled under laboratory containment level 2. Ethnicity: Black.

Human cervix epitheloid carcinoma

STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 12,14
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18

Preparation Note

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino AcidsNEAA + 10% Foetal Bovine Serum FBS / FCS.

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1.3x10,000 cells/cm² using 0.25% Trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.