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M2279

Minimum Essential Medium Eagle

With Earle′s salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Minimum Essential Medium Eagle
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Synonym(s):

EMEM, MEM

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500 mL
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$37.90
1 L
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$70.40
6 x 500 mL
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$197.00
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24 x 500 mL
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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

$37.90

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Product Name

Minimum Essential Medium Eagle, With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: yes
sodium pyruvate: no
L-glutamine: no
HEPES: no
NaHCO3: yes
Earle’s salts (5% CO2): yes

shipped in

ambient

storage temp.

2-8°C

General description

Minimum Essential Medium Eagle (MEM) is one of the most widely used synthetic cell culture media. It includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for the cultivation of a wide variety of cells grown in monolayers and attached cell lines, such as fibroblasts, in the presence of fetal bovine serum (FBS), calf, or horse sera.

Application

Minimum Essential Medium Eagle has been used to culture the:
  • explants of aortic valve leaflets
  • human liver cancer cell line (HepG2) cells[1]
  • human fetal brain progenitor-derived astrocyte cells (PDA)
  • breast cancer cell line (MCF-7)
  • human glioblastoma cell line (T98G)[2]

Preparation Note

Supplement with 0.292 g/L L-glutamine.

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This Item
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Minimum Essential Medium Eagle With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

M2279

Minimum Essential Medium Eagle

Minimum Essential Medium Eagle With Earle′s salts, non-essential amino acids and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

M5650

Minimum Essential Medium Eagle

Minimum Essential Medium Eagle Alpha Modification, With sodium bicarbonate and L-glutamine, without ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

M0200

Minimum Essential Medium Eagle

Minimum Essential Medium Eagle Alpha Modification, with ribonucleosides, deoxyribonucleosides and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

M8042

Minimum Essential Medium Eagle

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

liquid

form

liquid

form

liquid

form

liquid

components

phenol red: yes
sodium pyruvate: no
L-glutamine: no
HEPES: no
NaHCO3: yes
Earle’s salts (5% CO2): yes

components

phenol red: yes
NaHCO3: yes
L-glutamine: no
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes

components

phenol red: 0.011 g/L
glucose: 1.0 g/L
L-glutamine: 0.292 g/L
NaHCO3: 2.2 g/L

components

Hanks’ salts (2% CO2): no
glucose: yes
sodium pyruvate: yes
phenol red: yes
HEPES: no
L-glutamine: no
stable glutamine: no
Earle’s salts (5% CO2): yes
NaHCO3: yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
RNBL9391
RNBL9244
RNBL7911
RNBL7297
RNBL6717

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Minimum Essential Medium Eagle (MEM) Specifications
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Global Trade Item Number

SKUGTIN
M2279-10L04061837340598
M2279-20L04061837340604
M2279-500ML04061837847318
M2279-100ML04061834041771
M2279-1L04061834041788
M2279-24X500ML04061834041795
M2279-6X500ML04061834041818
M2279-6X1L04061834041801
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