KYSE-70

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 94072012, human esophagus, Epithelial

• Synonym(e): KY70 Cells, KYSE70 Cells
• UNSPSC Code: 41106514
• Biological source: human esophagus
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: Hypotriploid with 8% polyploidy
• Morphology: Epithelial
• Manufacturer/tradename: ECACC

Eigenschaften

• biological source: human esophagus
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 94072012-DNA-5UG, pkg of vial of cells 94072012-1VL
• growth mode: Adherent
• karyotype: Hypotriploid with 8% polyploidy
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

Biochem/physiol Actions

Established from a poorly differentiated invasive oesophageal squamous cell carcinoma resected from middle intra-thoracic oesophagus of a 77-year-old Japanese man prior to treatment. This cell line has been mycoplasma eradicated at ECACC. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line. It is a known phenomenon that SSC cell lines can lose their Y chromosome.

Human oesophageal squamous cell carcinoma

Preparation Note

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Split confluent cultures 1:5 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Doubling time ~35 hours.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.