KYSE-30

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 94072011, human esophagus, Polygonal

• Synonym(e): KYSE30 Cells
• UNSPSC Code: 41106514
• Biological source: human esophagus
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: Aneuploid
• Morphology: Polygonal
• Manufacturer/tradename: ECACC

Eigenschaften

• biological source: human esophagus
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 94072011-DNA-5UG, pkg of vial of cells 94072011-1VL
• growth mode: Adherent
• karyotype: Aneuploid
• morphology: Polygonal
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

Application

Study of human oesophageal cancer.

Biochem/physiol Actions

Human Asian squamous cell carcinoma

KYSE-30 was established from the oesophageal cancer of an untreated 64 year old male. The tumour sample was taken from the mucosal surface of a well differentiated squamous cell carcinoma. The cell line KYSE-30 was established with the use of tumours initially transplanted to athymic mice. The cells are reported to have a doubling time of 20.8 hrs in the exponential growth phase. A p53 mutation at the splice acceptor site of intron 6 and a 12 fold amplification of c-erb B has been reported. KYSE-30 cells express a large number of epidermal growth factor receptors, 1.2x10,000,000 sites/cell.

Preparation Note

RPMI 1640 + Ham′s F12 (1:1) + 2mM Glutamine + 2% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:10 i.e. seeding at 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.