OE33 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 96070808, human esophagus, Epithelial

• Synonym(e): JROECL33, JROECL33 Cells, OE-33 Cells
• UNSPSC Code: 41106514
• Biological source: human esophagus
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: Aneuploid
• Morphology: Epithelial
• Manufacturer/tradename: ECACC

Eigenschaften

• biological source: human esophagus
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 96070808-DNA-5UG, pkg of vial of cells 96070808-1VL
• growth mode: Adherent
• karyotype: Aneuploid
• morphology: Epithelial
• products: Not specified
• receptors: MHC class I, ICAM-1
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

Application

Study of oesophageal cancer

OE33 cell line has been used to study the effect of microRNA-MiR-193b on autophagy and cell death on these cells. It has also been used to study the sensitivity of the cells to anti-c-Met monoclonal antibody ABT-700.

Biochem/physiol Actions

Human Caucasian oesophageal carcinoma

The cell line OE33, also known as JROECL33, was established from the adenocarcinoma of the lower oesophagus (Barrett′s metaplasia) of a 73 year old female patient. The tumour was identified as pathological stage IIA (UICC) and showed poor differentiation. OE33 express HLA-A, -B and -C antigens (MHC class I) and ICAM-1 constitutively. Expression of HLA-DR (MHC class II) can be induced by treatment with interferon-gamma. The cells express epithelial cytokeratins and are tumourigenic in nude mice.

Preparation Note

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum FBS / FCS.

Split sub-confluent cultures (70-80%) 1:8 i.e. seeding at 1x10,000 cells/cm² using 0.25% Trypsin or Trypsin/EDTA; 5% CO₂; 37°C. Initially these cells grow slowly and can take up to 7 days until ready for the next split, 50% media changes will be necessary every 2-3 days (i.e. replacing half the old medium with fresh).

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.