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R0883

RPMI-1640 Medium

With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

RPMI-1640 Medium

Synonym(s):

Roswell Park Memorial Institute 1640 medium

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100 mL
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$17.90
500 mL
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$38.10
1 L
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$64.40
6 x 500 mL
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$194.00
6 x 1 L

Estimated to ship onJune 09, 2026fromMilliporeSigma Canada Ltd.

$334.00
24 x 500 mL
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$662.00

About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00217820

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Product Name

RPMI-1640 Medium, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Segment

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

sodium pyruvate: no
phenol red: yes
L-glutamine: no
NaHCO3: yes
HEPES: no

shipped in

ambient

storage temp.

2-8°C

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Application

RPMI-1640 Medium has been used:
  • to culture PC-3 prostate cell line and Jurkat cells[1]
  • to maintain a triple-negative MDA-MB-231 and a luminal type MCF7 human Caucasian breast adenocarcinoma cell line
  • to cultivate circulating tumor cells (CTCs)[2]

Preparation Note

Supplement with 0.3 g/L L-glutamine.

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RPMI-1640 Medium With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

R0883

RPMI-1640 Medium

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Sigma-Aldrich

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RPMI-1640 Medium With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

R8758

RPMI-1640 Medium

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

form

liquid

form

liquid

form

liquid

form

liquid

components

sodium pyruvate: no
phenol red: yes
L-glutamine: no
NaHCO3: yes
HEPES: no

components

sodium pyruvate: no
HEPES: 20 mM
L-glutamine: no
NaHCO3: yes
phenol red: yes

components

phenol red: no
L-glutamine: no
HEPES: no
sodium pyruvate: no
NaHCO3: yes

components

NaHCO3: yes
phenol red: yes
sodium pyruvate: no
HEPES: no
L-glutamine: yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
RNBM1122
RNBM0262
RNBM0101
RNBM0100
RNBL9396

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Protocols

Cell Viability and Proliferation XTT Assay Protocol Guide

Perform colorimetric assays for nonradioactive quantification of cellular proliferation, viability, and cytotoxicity for adherent or suspension cells cultured in 96-well microplates.

WST-1 Assay Protocol for Cell Viability

WST-1 assay protocol for cell viability and cytotoxicity measurements with preparation instructions, applications, FAQs, and troubleshooting.

MTT Assay Protocol for Cell Viability and Proliferation

MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.

View All Protocols

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RPMI-1640 Media
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Crohn's disease (CD), ulcerative colitis (UC) and inflammatory bowel disease (IBD) associated with primary sclerosing cholangitis (PSC-IBD), share three major pathogenetic mechanisms of inflammatory bowel disease (IBD)-gut dysbiosis, gut barrier failure and immune system dysregulation. While clinical differences among them
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Global Trade Item Number

SKUGTIN
R0883-6X1L04061836694067
R0883-6X500ML04061836694074
R0883-100ML04061836694036
R0883-20L04061837341441
R0883-10L04061837341434
R0883-1L04061838259943
R0883-500ML04061836694050
R0883-24X500ML04061836694043
1 Review This action will navigate to reviews.

Questions

  1. Could you tell me the exact concentration of glucose in this RPMI-1640 medium(cat#R0883 ), please ?

    1 answer

    1. For this RPMI formulation, the glucose concentration is 2 grams per liter.

      Please see the datasheet below which includes the formulations of all classical RPMI-1640 media products:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/229/770/r0883for.pdf

      Helpful?

Reviews

Active Filters

  1. Boston, MA
    • Reviews 3
    • Votes 12
    5 out of 5 stars.

    Great product!

    Reliable cell culture product that gets frequent use

    Helpful?