CHROP

Imprint^® Ultra Chromatin Optimization Kit

Kit designed to optimize sonication parameters for ChIP experiments

• NACRES: NA.54
• UNSPSC Code: 41116158

Eigenschaften

• Quality Segment: 200

Beschreibung

General description

The Imprint Ultra Chromatin Optimization Kit provides reagents needed to optimize chromatin sonication/shearing conditions before performing ChIP. It is imperative that chromatin is completely sheared into fragments with a peak around 500 base pairs (bp) for ChIP-qPCR assays, and < 500 bp for ChIP-Seq and ChIP-chip, for maximum specificity and resolution, and to ensure low background. For higher resolution applications like genome-wide location analysis by ChIP-chip or ChIP-Seq, sonication of input chromatin to below 500 bp, with a peak around 250 bp, is critical.

Other Notes

Sufficient reagents are provided to perform three sets of chromatin sonication optimization experiments. Each set would include five different conditions for sonication to optimize the parameters. Reagents are provided for a total of 15 reactions, including preparation of cell lysates from 10⁷ cells/reaction, nuclei lysis, sonication, cross-link reversal and DNA purification.

Legal Information

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

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Sicherheitshinweise

• pictograms: GHS02,GHS03,GHS08,GHS05,GHS07,GHS09
• signalword: Danger
• hcodes: H226,H271,H290,H302,H314,H334,H336,H410,EUH430
• target_organs: Central nervous system
• Lagerklasse: 5.1A - Strongly oxidizing hazardous materials
• flash_point_f: 100.4 °F
• flash_point_c: 38 °C
• pcodes: P201 - P202 - P210 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P391 - P405 - P501
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Ox. Liq. 1 - Resp. Sens. 1 - Skin Corr. 1A - STOT SE 3