D0550

3,5-Dinitrosalicylsäure

used in colorimetric determination of reducing sugars

• Synonym(e): 3,5-Dinitro-2-hydroxy-benzoesäure, DNS
• Lineare Formel: (O₂N)₂C₆H₂-2-(OH)CO₂H
• CAS-Nummer: 609-99-4
• Molekulargewicht: 228.12
• UNSPSC Code: 12171500
• NACRES: NA.47
• PubChem Substance ID: 24893243
• EC Number: 210-204-3
• Beilstein/REAXYS Number: 2220661
• MDL number: MFCD00007104
• eCl@ss: 32160703
• Assay: ≥98% (HPLC and titration)
• Form: powder

Eigenschaften

• Quality Segment: 200
• assay: ≥98% (HPLC and titration)
• form: powder
• mp: 168-172 °C (lit.)
• solubility: H₂O: 50 mg/mL
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: OC(=O)c1cc(cc(c1O)[N+]([O-])=O)[N+]([O-])=O
• InChI: 1S/C7H4N2O7/c10-6-4(7(11)12)1-3(8(13)14)2-5(6)9(15)16/h1-2,10H,(H,11,12)
• InChI key: LWFUFLREGJMOIZ-UHFFFAOYSA-N

Beschreibung

Application

3,5-Dinitrosalicylic acid (DNS) has been used to stop the reaction in in vitro α-amylase inhibition study. It has also been used for the quantitation of enzymatically released reducing sugars.

Biochem/physiol Actions

3,5-Dinitrosalicylic acid (DNS) is used in colorimetric determination of reducing sugars and to analyze glycosidase (glycoside hydrolase) activity by quantitation of enzymatically released reducing sugar. The dinitrosalicylic acid method has been compared to the Nelson-Somogi colorimetric method.

Sicherheitshinweise

• Piktogramme: GHS05,GHS07
• Signalwort: Danger
• Gefahrencodes: H302,H318
• Sicherheitshinweise: P264 - P270 - P280 - P301 + P312 - P305 + P351 + P338 - P501
• Hazard Classifications: Acute Tox. 4 Oral - Eye Dam. 1
• Lagerklasse: 4.1A - Other explosive hazardous materials
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): dust mask type N95 (US), Eyeshields, Gloves