D7006

Dihydrofolsäure

≥90%

• Synonym(e): 7,8-Dihydropteroyl-L-glutaminsäure
• Empirische Formel (Hill-System): C₁₉H₂₁N₇O₆
• CAS-Nummer: 4033-27-6
• Molekulargewicht: 443.41
• UNSPSC Code: 12352106
• NACRES: NA.79
• PubChem Substance ID: 24894068
• MDL number: MFCD00083619
• Beilstein/REAXYS Number: 69017
• Assay: ≥90%
• Form: powder

Eigenschaften

• biological source: synthetic (organic)
• Quality Segment: 200
• assay: ≥90%
• form: powder
• color: light yellow to dark yellow-orange
• storage temp.: −20°C
• SMILES string: NC1=NC(=O)C2=C(NCC(CNc3ccc(cc3)C(=O)N[C@@H](CCC(O)=O)C(O)=O)=N2)N1
• InChI: 1S/C19H21N7O6/c20-19-25-15-14(17(30)26-19)23-11(8-22-15)7-21-10-3-1-9(2-4-10)16(29)24-12(18(31)32)5-6-13(27)28/h1-4,12,21H,5-8H2,(H,24,29)(H,27,28)(H,31,32)(H4,20,22,25,26,30)/t12-/m0/s1
• InChI key: OZRNSSUDZOLUSN-LBPRGKRZSA-N
• Gene Information: human ... DHFRP1(573971) ; mouse ... Dhfr(13361)

Beschreibung

General description

Intermediate in mammalian conversion of dietary folic acid to tetrahydrofolate by dihydrofolate reductase (DHFR). In bacteria, dihydrofolic acid is generated from 7,8-dihydropteroate by dihydrofolate synthetase.

Application

Dihydrofolic acid has been used in diaphorase-coupled assay for dihydrofolate reductase (DHFR) activity. It has also been used as a component in assay buffer to determine dihydrofolate reductase (DHFR) activity.

Biochem/physiol Actions

Folic acid (FA) and dihydrofolic acid (FAH2) are substrates of dihydrofolate reductase(s) which reduce them to tetrahydrofolate (THF), which in turn supports ‘one carbon′ transfer. Tetrahydrofolates are required for de novo synthesis of purines, thymidylic acid and various amino acids and for post-translational methylation (epigenetics).

Packaging

Verschweisste Ampulle

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• Gefahrencodes: H315,H319,H335
• Sicherheitshinweise: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Zielorgane: Respiratory system
• Lagerklasse: 11 - Combustible Solids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): dust mask type N95 (US), Eyeshields, Gloves