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D2650

Dimetilsulfóxido

Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%

Dimethyl sulfoxide

Sinónimos:

DMSO

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A ustedes/SKUDisponibilidadPrecio
100 mL

Disponible para enviar HOYdesdeToluca

MXP 6,229.00

Acerca de este artículo

Fórmula lineal:
(CH3)2SO
Número CAS:
Peso molecular:
78.13
UNSPSC Code:
12191502
NACRES:
NA.75
PubChem Substance ID:
EC Number:
200-664-3
Beilstein/REAXYS Number:
506008
MDL number:
Assay:
≥99.7%
Grade:
Hybri-Max
Technique(s):
cell culture | hybridoma: suitable, cryopreservation: suitable
Bp:
189 °C (lit.)
Vapor pressure:
0.42 mmHg ( 20 °C)

MXP 6,229.00


Disponible para enviar HOYDetalles


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Servicio técnico
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grade

Hybri-Max

Quality Segment

vapor density

2.7 (vs air)

vapor pressure

0.42 mmHg ( 20 °C)

sterility

sterile-filtered

product line

BioReagent

assay

≥99.7%

form

liquid

autoignition temp.

573 °F

feature

subscription eligible

expl. lim.

42 %, 63 °F

technique(s)

cell culture | hybridoma: suitable, cryopreservation: suitable

impurities

endotoxin, tested, ≤0.1% water
water

refractive index

n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

solubility

H2O: miscible (completely)

density

1.10 g/mL (lit.)

SMILES string

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

Application

El DMSO es un disolvente aprótico polar utilizado en reacciones químicas, en reacciones en cadena de la polimerasa (PCR) y como agente de vitrificación crioprotector para la conservación de células, tejidos y órganos. El DMSO se utiliza en los medios de congelación celular para proteger a las células de las lesiones mecánicas inducidas por el cristal de hielo. Se utiliza para el almacenamiento en congelación de líneas celulares de hibridomas y heteroploides primarias, subcultivadas y recombinantes; células madre embrionarias (ESC) y células madre hematopoyéticas. El DMSO se utiliza con frecuencia en combinación con la seroalbúmina bovina (BSA) o el suero bovino fetal (FBS).

Packaging

This product features a liner in the cap designed to prevent leakage. In rare cases, the liner may remain on the neck of the bottle after cap removal. This does not affect the product′s integrity. If this occurs, simply reattach the liner to the cap.
100 ml en botella de vidrio
El embalaje de ampollas de vidrio está ahora disponible bajo un nuevo número de referencia, D2653

Other Notes

Este producto es un producto Hybri-Max. Se analiza en hibridomas y se evalúa su idoneidad para la congelación celular. Este producto está esterilizado mediante filtración (0,2 micras) y probado para determinar la concentración de endotoxinas.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Disclaimer

Fácilmente superenfriado, vuelve a fundir lentamente a temperatura ambiente. Producto solidificado que puede volver a licuarse por calentamiento a temperatura ambiente sin detrimento del producto.

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Este artículo
D2653V900090D4540
technique(s)

cell culture | hybridoma: suitable, cryopreservation: suitable

technique(s)

cell culture | hybridoma: suitable, cryopreservation: suitable

technique(s)

-

technique(s)

cell culture | plant: suitable, cryopreservation: suitable

assay

≥99.7%

assay

≥99.7%

assay

99%

assay

≥99.5% (GC)

Quality Level

400

Quality Level

300

Quality Level

-

Quality Level

200

grade

Hybri-Max

grade

Hybri-Max

grade

reagent grade

grade

-

solubility

H2O: miscible (completely)

solubility

H2O: miscible (completely)

solubility

-

solubility

H2O: miscible (completely)

form

liquid

form

liquid

form

liquid

form

liquid


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Clase de almacenamiento

10 - Combustible liquids

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter



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Certificados de análisis (COA)

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Questions

1–10 of 21 Questions  
  1. Is this DMSO suitable for HPLC?

    1 answer
    1. This DMSO product has not been tested for suitability in HPLC applications. Please see the links below to review DMSO products suitable for HPLC:
      https://www.sigmaaldrich.com/product/sigald/34869
      https://www.sigmaaldrich.com/product/saj/074871
      https://www.sigmaaldrich.com/product/sial/94563

      Helpful?

  2. Is this DMSO suitable to be used as a drug solvent in animal studies?

    1 answer
    1. This product has not been tested for use in animal models. However, this is a sterile-filtered DMSO that has been tested for cytotoxicity and is expected to be suitable for in vivo research applications.

      Helpful?

  3. How many days after opening the bottle is DMSO safe to use?

    1 answer
    1. This product is assigned an expiration date of approximately two years from the date of Quality Release. This date is lot specific and available on the product label and Certificate of Analysis. After opening, the material should be stored tightly sealed to prevent moisture uptake. Given proper handling and storage, the product should be stable until the published expiration date.

      Helpful?

  4. I ordered 10 x 10ml vials for fibroblast culture work. My first endeavor in culture domain. What are the storage conditions for DMSO of this grade? This arrived in plain cardboard box at room temperature.

    1 answer
    1. DMSO is hygroscopic and light-sensitive. The storage container must protect from light and moisture uptake. Since this DMSO is sterile-filtered and intended for cell culture applications, the storage containers must also be sterile and endotoxin-free. It is recommended to purge aliquots with inert gas and store them in a compatible, tightly sealed container, at a cool storage area, such as 4°C. DMSO is not compatible with many types of plastics, so it is advisable to store it in appropriate amber glass vials for long-term storage. Please refer to the Safety Data Sheet and the Product Information Sheet for compatibility of DMSO with various materials and for further details.

      Helpful?

  5. Is it safe in terms of health and product safety to store a leftover DMSO ampule covered with parafilm in a flammable cabinet?

    1 answer
    1. DMSO is hygroscopic and light-sensitive. The storage container must protect from light and moisture uptake. Since this DMSO is sterile-filtered and intended for cell culture applications, the storage containers must also be sterile and endotoxin-free. It is recommended to purge aliquots and store them under inert gas, in a compatible, tightly sealed container, at a cool storage area, such as 4°C.

      DMSO is not compatible with many types of plastics, so it is advisable to store it in appropriate amber glass vials for long-term storage. Please refer to the Product Information Sheet and the Safety Data Sheet at the link below for compatibility of DMSO with various materials and for further details:

      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/944/265

      Helpful?

  6. Is this anhydrous?

    1 answer
    1. This product is not considered anhydrous. The maximum allowable water content is 0.1% which is reported on the lot-specific Certificate of Analysis. Please see the link below to review the anhydrous DMSO products available:
      https://www.sigmaaldrich.com/search/dmso-anhydrous?focus=products&page=1&perpage=30&sort=relevance&term=DMSO%20anhydrous&type=product

      Helpful?

  7. I ordered 10 ml glass vials, used 7 ml, Can I store it in centrifuge tubes? For how long can I use that?

    1 answer
    1. It may be best to store DMSO in amber glass vials for long term storage, as this compound is not compatible with many types of plastics. The compatibility of DMSO with various materials is listed below:
      Compatible: LDPE, HDPE, polypropylene, PPCO(polypropylene copolymer), polymethylpentene, nylon, teflon FEP
      Moderately compatible: polystyrene, ECTFE/ETFE
      Incompatible: polysulfone, flexible and rigid PVC tubing, polycarbonate

      Note that DMSO is hygroscopic and light sensitive. The storage container selected must offer protection from light and moisture uptake. The product is assigned a firm expiration date. Please refer to the lot specific Certificate of Analysis to assume the material is within the expected shelf life.

      Helpful?

  8. E' solo ad uso per ricerca o utilizzabile anche in diagnostica/human? Grazie

    1 answer
    1. This product is not suitable for diagnostic/human use.

      Helpful?

  9. Is this product packaged in a bottle with a septa cap?

    1 answer
    1. No, this product is not packaged with a septa cap. It comes in a 125 ml amber glass bottle with a 28 mm solid white cap.

      Helpful?

  10. Can I use it for PCR?

    1 answer
    1. While this product is tested specifically for use in cell culture, there is no reason to suspect that it would not perform well in PCR applications. The product is NOT tested for amplification, DNAse, or nuclease activity. Should this testing be important, see the link below to product options that include this testing:
      D9170 DMSO PCR Reagent:
      https://www.sigmaaldrich.com/product/sigma/d9170
      D8418 DMSO Molecular Biology Tested
      https://www.sigmaaldrich.com/US/en/product/sigma/d8418

      Helpful?

1–10 of 21 Questions  

Reviews

Active Filters

  1. Turkey
    • Review 1
    • Votes 577
    5 out of 5 stars.

    Is this product already concentration adjusted

    Is this product already concentration adjusted, as you mentioned this product used %8.7 concentration bu the thing i cannot understand that is this item already diluted for ready to use or do we have to dilute it what concentration we need ?

    Helpful?

  2. 9 Ratings-only reviews