DIF001

3T3-L1 DIFFERENTIATION KIT

Sufficient for 100 mL differentiation medium

• Synonym(e): 3T3-L1 Differentiation Kit
• NACRES: NA.75
• UNSPSC Code: 12352207
• eCl@ss: 32040201

Eigenschaften

• form: solution
• usage: 100 mL differentiation medium
• technique(s): cell culture | mammalian: suitable
• shipped in: wet ice
• storage temp.: −20°C

Beschreibung

General description

3T3-L1 cells are derived from mouse 3T3 cells and provide a widely-used fundamental model in the study of adipose physiology and metabolic diseases. They exhibit a fibroblast-like morphology before differentiation but become rounded and accumulate lipid droplets several days after the initiation of differentiation. The accumulated lipid droplets can be visualized by light microscopy. The in vitro differentiated 3T3-L1 adipocytes result in characteristics similar to tissue-derived adipocytes and have been commonly used to study adipogenesis, lipolysis, and metabolic dysfunctions. The 3T3-L1 Differentiation Kit provides enough supplements to make 100 ml of differentiation medium and 600 ml of maintenance medium which is sufficient material for 12 100 mm culture dishes.

Application

3T3-L1 DIFFERENTIATION KIT has been used in:

• Differentiation of 3T3-L1 preadipocytes to adipocytes.
• Study of obesity, adipogenesis, lipolysis and lipid metabolism
• Differentiation of 3T3-L1 preadipocytes to adipocytes
• Study of obesity, adipogenesis, lipolysis and lipid metabolism.

Preparation Note

Insulin (1.5 mg/ml) 0.6 ml (Catalog Number DIF001A, Green Cap)
Differentiation Cocktail, 1000x (lyophilized) 1 vl (Catalog Number DIF001B, Yellow Cap)
DMSO (anhydrous) 0.5 ml (Catalog Number DIF001C, Blue Cap)

Store the kit at −20 °C, protected from light.

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• Gefahrencodes: H360FD
• Sicherheitshinweise: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Repr. 1B
• Lagerklasse: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3