E1388

β-Endothelial Cell Growth Factor human

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(e): ECGS, β-ECGF
• CAS-Nummer: 105843-51-4
• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD00131012
• Biological source: human
• Recombinant: expressed in E. coli
• Assay: ≥97% (SDS-PAGE)
• Form: lyophilized powder
• Mol wt: predicted mol wt ~17 kDa
• Impurities: ≤1.0 EU/μg protein Endotoxin level (LAL test)

Eigenschaften

• Produktname: β-Endothelial Cell Growth Factor human, β-ECGF, recombinant, expressed in E. coli, suitable for cell culture
• biological source: human
• Quality Segment: 200
• recombinant: expressed in E. coli
• assay: ≥97% (SDS-PAGE)
• form: lyophilized powder
• potency: 0.03-0.3 ng/mL ED₅₀/EC₅₀
• quality: endotoxin tested
• mol wt: predicted mol wt ~17 kDa
• packaging: pkg of 25 μg
• storage condition: avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1.0 EU/μg protein Endotoxin level (LAL test)
• UniProt accession no.: P49765
• storage temp.: −20°C
• Gene Information: human ... VEGFB(7423)

Beschreibung

General description

β‑Endothelial Cell Growth Factor (β‑ECGF) is a recombinant human protein expressed in Escherichia coli and supplied as a lyophilized powder suitable for mammalian cell culture. The β‑ECGF gene encodes an endothelial growth factor and is mapped to human chromosome 11q13.1. Recombinant β‑ECGF consists of a 14‑amino‑acid N‑terminal extension of acidic fibroblast growth factor. After removal of the initiation methionine, the protein comprises 154 amino acids with a predicted molecular weight of approximately 17 kDa.

Application

Recombinant human β‑Endothelial Cell Growth Factor (β‑ECGF) has been used in a range of endothelial research applications, including:

• Endothelial cell culture studies
• Human umbilical vein endothelial cell (HUVEC) culture
• Cell death and viability assays in in vitro model systems

Biochem/physiol Actions

β‑Endothelial Cell Growth Factor (β‑ECGF) is a potent mitogen for endothelial cells, with mitogenic activity reported to be enhanced in the presence of heparin. In vitro, β‑ECGF stimulates the growth of quiescent human umbilical vein endothelial cells (HUVECs). β‑ECGF has been associated with endothelial‑mediated lipid and glucose metabolism pathways and has been studied extensively in cellular and molecular research models.

Physical form

Lyophilized from a 0.2 μm filtered solution in 10 mM MOPS, 50 mM ammonium sulfate, 0.2 mM DTT, and 0.2 mM EDTA, pH 7.2, containing 50 μg bovine serum albumin per 1 μg cytokine.

Analysis Note

The proliferative activity is tested in culture by using quiescent NR6R-3T3 fibroblasts.

Other Notes

14 amino acid, N-terminal extended form of human FGF-acidic.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, type N95 (US)
• Signalwort: Danger
• Gefahrencodes: EUH451
• Sicherheitshinweise: P201 - P202 - P273 - P391 - P501
• Hazard Classifications: vPvM