Histone Deacetylase 8 (HDAC8) Inhibitor Screening Kit

Name: Histone Deacetylase 8 (HDAC8) Inhibitor Screening Kit
Brand: SIGMA

100 assays in 96 well plates

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Über diesen Artikel

EG-Nummer:

3.5.1.98 (BRENDA, IUBMB)

NACRES:

NA.41

UNSPSC Code:

41116158

Usage:

100 assays in 96 well plates

Storage temp.:

−20°C

Shipped in:

wet ice

Technischer Dienst

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Unterstützung erhalten

Eigenschaften

usage

100 assays in 96 well plates

NCBI accession no.

NM_001166418 (Human), NM_027382 (Mouse)

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... HDAC8(55869)
mouse ... HDAC8(70315)

Beschreibung

General description

Histone deacetylases (HDACs) are a large family of enzymes that remove acetyl groups from histone proteins. Site specific histone acetylation and deacetylation have been shown to activate or repress eukaryotic gene transcription, respectively, and as a consequence, HDACs play a crucial role in mammalian development and disease. HDACs are involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence.

With Sigma′s HDAC8 Inhibitor Screening Kit, HDAC8 Enzyme acts with the supplied Developer to deacetylate and then cleave the HDAC8 Substrate (R-H-K(Ac)-K(Ac)-AFC). This activity releases the quenched fluorescent group, AFC, which can be detected at Em/Ex=380/500 nm. In the presence of a HDAC8 inhibitor, AFC is not released and its fluorescence remains quenched. The kit provides a rapid, simple, sensitive, and reliable test, suitable for either individual tests or high throughput screening of HDAC8 inhibitors. Trichostatin A (TSA) is included as a control inhibitor to compare with the efficacy of test inhibitors.