F0500

Fast Blue RR Salz

crystalline

• Synonym(e): 4-Benzoylamino-2,5-dimethoxy-benzoldiazoniumchlorid Zink Doppelsalz, Diazotiertes 4-Amino-2,5-dimethoxy-benzanilid
• Lineare Formel: C₁₅H₁₄ClN₃O₃ · 1/2 ZnCl₂
• CAS-Nummer: 14726-29-5
• Molekulargewicht: 387.89
• NACRES: NA.47
• PubChem Substance ID: 24894722
• UNSPSC Code: 12171500
• EC Number: 238-777-5
• MDL number: MFCD00074762
• Colour Index Number: 37155

Eigenschaften

• Produktname: Fast Blue RR Salz, crystalline
• form: crystalline
• Quality Segment: 200
• technique(s): microbe id | staining: suitable
• solubility: water: 1 mg/mL, clear to hazy, yellow-green
• ε (extinction coefficient): ≥15000 at 326-332 nm, ≥7000 at 390-396 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: −20°C
• SMILES string: [Cl-].[Cl-].Cl[Zn]Cl.COc1cc([N+]#N)c(OC)cc1NC(=O)c2ccccc2.COc3cc([N+]#N)c(OC)cc3NC(=O)c4ccccc4
• InChI: 1S/2C15H13N3O3.4ClH.Zn/c2*1-20-13-9-12(18-16)14(21-2)8-11(13)17-15(19)10-6-4-3-5-7-10;;;;;/h2*3-9H,1-2H3;4*1H;/q;;;;;;+2/p-2
• InChI key: LLARVKAOQLUCQI-UHFFFAOYSA-L

Beschreibung

Application

Fast blue RR salt has been used in the immunohistochemistry to detect the activity of alkaline phosphatase. It has been used in the procedure of connective tissue staining using picrosirius red.

Biochem/physiol Actions

Fast blue RR is mainly used in histological stainings. It binds to the reaction product of alkaline phosphatase and precipitates it as an insoluble compound. The sites of alkaline phosphatase activity appears black. Fast blue RR is also used for the detection of esterase activity. It couples with naphthol (released from naphthol acetate) and forms an insoluble azo-dye which appears red/blue.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, type N95 (US)
• Piktogramme: GHS07
• Signalwort: Warning
• Gefahrencodes: H319
• Sicherheitshinweise: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2