Lipopolysaccharide aus Escherichia coli O111:B4

Name: Lipopolysaccharide aus <I>Escherichia coli</I> O111:B4
Brand: SIGMA

FITC conjugate

Synonym(e):

LPS

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Über diesen Artikel

CAS-Nummer:

93572-42-0

UNSPSC Code:

12352201

EC Number:

297-473-0

NACRES:

NA.25

MDL number:

MFCD00164401

Technischer Dienst

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Unterstützung erhalten

Eigenschaften

biological source

Escherichia coli (O111:B4)

Quality Segment

200

conjugate

FITC conjugate

form

lyophilized powder

extent of labeling

2-10 μg FITC per mg LPS

color

yellow to orange

solubility

H₂O: soluble 4.90-5.10 mg/mL, faintly hazy to hazy, pale yellow to yellow

storage temp.

2-8°C

Beschreibung

Application

FITC-Lipopolysaccharides (FITC-LPS) may be used to study processes such as the movement/transport of LPS across tissue barriers such as the colonic epithelium and cellular binding and internalization of lipopolysaccharides with fluorescence-based assay systems. It may be used to evaluate, qualify and quantify lipopolysaccharides (LPS) removal reagents and procedures. FITC-LPS conjugate may be used for the detection of LPA binding proteins and motifs.

Lipopolysaccharide (LPS) sind charakteristische Bestandteile der Zellwand von gramnegativen Bakterien. LPS und seine Lipid-A-Gruppe stimulieren Zellen des angeborenen Immunsystems durch den Toll-like-Rezeptor 4 (TLR4). TLR4 gehört zur Toll-like-Rezeptor-Proteinfamilie, die häufige Pathogen-assoziierte molekulare Strukturen (PAMPs) erkennt.

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Preparation Note

The product is soluble in water (5 mg/ml) yielding a hazy, yellow to orange solution. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution. For cell culture use, LPS should be reconstituted by adding 1 ml of sterile balanced salt solution or cell culture medium to a vial (1 mg) and swirling gently until the powder dissolves. Solutions can be further diluted to the desired working concentration with additional sterile balanced salt solutions or cell culture media.

Other Notes

To gain a comprehensive understanding of our extensive range of lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.