FG0100

Triglycerid- und freie Glycerin-Kits und -Reagenzien

1 kit sufficient for 1000 reactions

• Synonym(e): Triglyceride and Free Glycerol Kits and Reagents
• NACRES: NA.26
• UNSPSC Code: 12352202

Eigenschaften

• Quality Segment: 200
• usage: kit sufficient for 1000 reactions
• detection method: colorimetric
• storage temp.: 2-8°C

Beschreibung

General description

Glycerol, also referred to as glycerin or glycerine, is a 3 carbon sugar alcohol that forms the backbone of fatty acids. It is a central component for synthesis of all lipids, and acts as a backbone for triglycerides and phospholipids, which play an important role in cell membrane structure. Due to its low toxicity, glycerol is widely used in pharmaceutical, food and cosmetic formulations and is the main waste by-product of biodiesel production via transesterification. In addition to kits, the individual reagents and glycerol standard are available separately when fewer reactions are needed.

Application

The Free Glycerol Determination Kit has been used to determine the level of glycerol released into the medium from mammalian cells grown in vitro .

The Free Glycerol Determination Kit measures free, endogenous glycerol using coupled enzyme reactions and does not include initial lipase hydrolysis. Glycerol is phosphorylated by adenosine-5′-triphosphate (ATP) forming glycerol-1-phosphate (G-1-P) and adenosine-5′-diphosphate (ADP) in the reaction catalyzed by glycerol kinase (GK). G-1-P is then oxidized by glycerol phosphate oxidase (GPO) to dihydroxyacetone phosphate (DAP) and hydrogen peroxide (H₂O₂). Peroxidase catalyzes the coupling of H₂O₂ with 4-aminoantipyrine (4-AAP) and sodium N-ethyl-N-(3-sulfopropyl) m-anisidine (ESPA) to produce a quinoneimine dye that shows an absorbance maximum at 540 nm. The increase in absorbance at 540 nm is directly proportional to the free glycerol concentration of the sample.

Das Triglycerid-Kit und das Kit für freies Glycerin dienen zur quantitativen Bestimmung von Glycerin, der Gesamtzahl an Triglyceriden bzw. von freien Triglyceriden.

Suitable for the quantitative enzymatic determination of glycerol in serum or plasma.

Other Notes

Zusätzlich zu den Kits sind die einzelnen Reagenzien und der Glycerinstandard separat erhältlich für den Fall, dass weniger Reaktionen benötigt werden.

Sicherheitshinweise

• Piktogramme: GHS05,GHS07,GHS09
• Signalwort: Danger
• Gefahrencodes: H302,H315,H318,H410
• Sicherheitshinweise: P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2
• Lagerklasse: 10 - Combustible liquids