G0774

Glycerokinase from Bacillus stearothermophilus

buffered aqueous solution, ≥75 units/mg protein (biuret)

• Synonym(e): ATP:glycerol 3-phosphotransferase, Glycerol Kinase
• CAS-Nummer: 9030-66-4
• UNSPSC Code: 12352204
• eCl@ss: 32160410
• EC Number: 232-862-0
• NACRES: NA.54
• MDL number: MFCD00131205
• EG-Nummer: 2.7.1.30 (BRENDA, IUBMB)
• Specific activity: ≥75 units/mg protein (biuret)
• Assay: ≥5.0 mg protein/mL (biuret)
• Biological source: Bacillus sp. (Bacillus steaarothermophilus)

Eigenschaften

• biological source: Bacillus sp. (Bacillus steaarothermophilus)
• Quality Segment: 200
• assay: ≥5.0 mg protein/mL (biuret)
• form: buffered aqueous solution
• specific activity: ≥75 units/mg protein (biuret)
• storage condition: dry at room temperature
• color: beige
• application(s): life science and biopharma
• storage temp.: 2-8°C
• Gene Information: Bacillus sp. ... glpK(89613566)

Beschreibung

General description

Research area: Cell Signaling

Glycerol kinase is encoded by the GK gene on chromosome Xp21.2. Glycerol kinase is predominantly active in the liver.

Application

Glycerokinase from Bacillus stearothermophilus has been used in steady-state kinetics studies.

Biochem/physiol Actions

Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. It is also subject to feedback regulation by fructose-1,6-bisphosphate.Oxidized glycerol-3-phosphate results in dihydroxyacetone phosphate which enters either glycolysis or gluconeogenesis. Elevated glycerol is observed in glycerol kinase deficiency leading to pseudohypertriglyceridemia.

Physical form

Stabilized solution in Tris buffer, pH 7.3

Other Notes

One unit will convert 1.0 μmole of glycerol and ATP to L-α-glycerophosphate and ADP per min at pH 9.8 at 25 °C in a coupled system with PK/LDH.

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• Gefahrencodes: H334
• Sicherheitshinweise: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, type N95 (US)