G4259

β-Glucuronidase from Helix aspersa (garden snail)

Type HA-4

• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-606-8
• MDL number: MFCD00130629
• EG-Nummer: 3.2.1.31 (BRENDA, IUBMB)
• Specific activity: ≥300,000 units/g solid solid

Eigenschaften

• type: Type HA-4
• Quality Segment: 200
• form: partially purified powder
• specific activity: ≥300,000 units/g solid solid
• secondary activity: ≤7,500 units/g solid sulfatase
• solubility: H₂O: soluble 1.90-2.10 mg/mL, clear to slightly hazy
• application(s): clinical testing
• storage temp.: −20°C

Beschreibung

Application

β-glucuronidase was used in enzymic hydrolysis of tissue homogenates for liquid chromatography-electrospray ion trap mass spectrometry (LC/MSⁿ) analysis, to study the structures of degradation products of baicalin.

Biochem/physiol Actions

β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.

Other Notes

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37°C at the pH 5.0 (30 min assay).

One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• Gefahrencodes: H334
• Lagerklasse: 11 - Combustible Solids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, type N95 (US)
• Sicherheitshinweise: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• WGK: WGK 1