G7907

Galactose-Oxidase aus Dactylium dendroides

≥30 units/mg solid

• Synonym(e): D-Galactose:oxygen 6-oxidoreductase
• CAS-Nummer: 9028-79-9
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-843-7
• MDL number: MFCD00131118
• EG-Nummer: 1.1.3.9 (BRENDA, IUBMB)
• Specific activity: ≥30 units/mg solid
• Biological source: fungus (Dactylium dendroides)

Eigenschaften

• biological source: fungus (Dactylium dendroides)
• Quality Segment: 200
• form: lyophilized
• specific activity: ≥30 units/mg solid
• storage temp.: −20°C

Beschreibung

General description

Galactose oxidase is an extracellular copper-containing enzyme, secreted by the deuteromycete fungus Dactylium dendroides. It catalyzes the oxidation of a range of primary alcohols, including D-galactose, to the corresponding aldehyde, with reduction of oxygen to hydrogen peroxide.

Galactose oxidase is a member of radicalcoupled copper oxidases family. It is a fungal secretory enzyme.

Application

Galactose oxidase may be used as an analytical tool for the specific determination of D-galactose in blood plasma, plant extracts, and phospholipids. It could be used for the characterization of terminal D-galactoside units in several polymers. It may also be useful in the determination of lactose.

Galactose Oxidase from Dactylium dendroides has been used as a component for galactose oxidase treatment of arabinogalactan. It has also been used to co-immobilise with peroxidase for the preparation of a biosensor for galactose detection.

Biochem/physiol Actions

Galactose oxidase catalyzes the coversion of D-galactose to D-galacto-hexodialdose.
2-Deoxy-D-galactose, lactose, melibiose, raffinose and stachyose react with galactose oxidase in the peroxidase:o-tolidine system.
Essentially no oxidation of D-glucose, L-galactose, L-arabinose or D-glucuronate has been observed.

The specificity for galactose and other sugars is similar to that described by Avigad.

Galactose oxidase has several applications in bioanalytics and histology. This free radical enzyme possess high substrate specificity.

Physical form

Lyophilized, contains buffer salts and stabilizer

Preparation Note

Chromatographically purified

Other Notes

One unit will produce a ΔA₄₂₅ of 1.0 per min at pH 6.0 at 25 °C, in a peroxidase and o-tolidine system. Reaction volume = 3.4 mL. Light path = 1 cm.

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• Gefahrencodes: H334
• Sicherheitshinweise: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable