GS313F

HyStem^®-C Thiol-Modified Hyaluronan and Gelatin Hydrogel Kit

• Synonym(e): HyStem^®-C Thiol-Modified Hyaluronan and Gelatin Hydrogel Kit, 3D hydrogel, 3d cell culture, PEGDA, collagen, hyaluronic acid, hydrogel, matrigel
• NACRES: NA.51

Eigenschaften

• storage temp.: 2-8°C

Beschreibung

General description

HyStem^®-C Hydrogel Kits - The starter matrix. HyStem^®-C hydrogels provide an excellent starting point for optimizing the matrix for cell culture. HyStem-C is fully chemically-defined and based on three biocompatible components: thiol-modified hyaluronan (Glycosil), thiol-reactive crosslinker, PEGDA (Extralink), and thiol-modified denatured collagen (Gelin-S). Gelin-S provides basic cell-attachment sites for a wide variety of primary cells and cell lines and is therefore recommended as an ideal substrate for adherent cell types and for cell culture optimization. In some cases, HyStem-C hydrogels can be further enhanced by the addition of ECM proteins to match native signals. Product may be shipped ambient, but please store components at the temperature stated on the vials until ready for use.

Application

GS313F replaces Sigma HYSC020 with a new user-friendly buffering sytem. Follow preparation instructions found in the product information sheet.

Features and Benefits

Features - Hydrogels are suitable for culturing primary cells, stem cells and cell lines. - Cells can be encapsulated or grown on the hydrogel surface in any format, including culture flasks, 6- to 384-well plates or tissue culture inserts. - Hydrogels can be easily customized by the user to possess the desired stiffness and gelation time by manipulating component concentration and mixing ratios. - Customizable gelation properties including gelation time and hydrogel stiffness.

Gelation Reconstituted HyStem-C components remain liquid at 15 to 37°C. The hydrogel is formed when the crosslinking agent, Extralink^® (PEGDA) is added to a mixture of Glycosil(thiol-modified hyaluronan) and Gelin-S (thiol-modified gelatin). Gelation occurs in about twenty minutes after all three components are mixed. No steps depend on low temperatures or low pH. Diluting the components with phosphate-buffered saline (PBS) or cell-culture medium can increase the gelation time.

3D Cell Recovery Matrix For digesting the matrix and recovering cells, we recommend using a Collagenase/Hyaluronidase mixture.

Legal Information

Extralink is a registered trademark of Glycosan BioSystems, Inc.

Hystem is a registered trademark of BIOTIME, INC

Sicherheitshinweise

• Piktogramme: GHS05,GHS07
• Signalwort: Danger
• Gefahrencodes: H315,H317,H318
• Sicherheitshinweise: P261 - P264 - P272 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1
• Lagerklasse: 10 - Combustible liquids
• Was ist los?: WGK 3