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H7782

Hybridization solution

Synonym(e):

Hyb solution, Hybridization solution for in situ hybridization, Ready-to-use hybridization solution

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Größe/SKUVerfügbarkeitPreis
6 mL
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€ 106,00

Über diesen Artikel

UNSPSC Code:
12161503
EC Number:
200-842-0
NACRES:
NA.32

€ 106,00


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form

liquid

Quality Segment

technique(s)

FISH: suitable, nucleic acid detection: suitable

storage temp.

−20°C

General description

In situ hybridization assays detect a specific nucleic acid sequence of a gene at the cellular level. Hybridization solution is used to enhance hybrid formation between the DNA probe and the target mRNA on tissue sections. It may be used for reconstituting and/or diluting the probe for in situ hybridization experiments, or as a negative control (without probe).
Ready-to-use hybridization solution optimized for in situ hybridization (ISH) procedures.

Application

Hybridization solution has been used in:
  • fluorescent in situ hybridization (FISH) to perform probe annealing
  • in situ hybridization (ISH) to dilute probe for the detection of amastigote forms of Leishmania infantum
  • FISH analysis to hybridize probes


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Dieser Artikel
H7140P1415H7033
description

-

description

for Northern and Southern blotting, liquid (2x concentrate)

description

for Northern and Southern blotting, liquid (2x concentrate)

description

for Northern and Southern blotting, solution

technique(s)

nucleic acid detection: suitable, FISH: suitable

technique(s)

nucleic acid detection: suitable

technique(s)

nucleic acid detection: suitable

technique(s)

nucleic acid detection: suitable

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

200

form

liquid

form

liquid (2x concentrate)

form

liquid (2x concentrate)

form

solution

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-10 to -25°C

storage temp.

room temp


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Piktogramme

Health hazard

Signalwort

Danger

Gefahrencodes

Hazard Classifications

Carc. 2 - Repr. 1B - STOT RE 2 Oral

Zielorgane

Blood

Lagerklasse

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Zulassungslistungen

Zulassungslistungen werden hauptsächlich für chemische Produkte erstellt. Für nicht-chemische Produkte können hier nur begrenzte Angaben gemacht werden. Kein Eintrag bedeutet, dass keine der Komponenten gelistet ist. Es liegt in der Verantwortung des Benutzers, die sichere und legale Verwendung des Produkts zu gewährleisten.

75-12-7

CAS No.

75-12-7

CAS No.


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Analysenzertifikate (COA)

Lot/Batch Number

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Questions

1–4 of 4 Questions  
  1. Does this hybridization mix contain 50% formamide?

    1 answer
    1. This product contains formamide, with the exact composition being proprietary. The Safety Data Sheet indicates that the formamide content is greater than or equal to 50% and less than 70%.

      Helpful?

  2. Is this buffer appropriate for Fluorescent in situ hybridization using probes labeled with ATTO by nick translation?

    1 answer
    1. This product has not been tested for the application. So, there is no reference data available.

      Helpful?

  3. Is this buffer appropriate to use with all probe types? Will it work with labeled (DIG, DNP, Biotin, etc.) polynucleotide probes?

    1 answer
    1. The buffer is optimized for both DNA and RNA probes for in situ hybridization. It has been used with FITC and biotinylated probes.

      Helpful?

  4. What is the composition of the Hybridization solution ( H7782)?

    1 answer
    1. The specific formulation of this product is considered proprietary but includes dextran sulfate and formamide in a sodium citrate solution.

      Helpful?

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