H9166

Vascular Endothelial Growth Factor 165 human

VEGF165, recombinant, expressed in HEK 293 cells, HumanKine^®, suitable for cell culture

• Synonym(e): Endothelial Growth Factor, Vascular Growth Factor
• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD00286542
• Biological source: human
• Recombinant: expressed in HEK 293 cells
• Assay: >95% (SDS-PAGE)
• Form: lyophilized powder
• Mol wt: dimer 45 kDa (glycosylated)
• Impurities: ≤1 EU/μg

Eigenschaften

• biological source: human
• Quality Segment: 100
• recombinant: expressed in HEK 293 cells
• assay: >95% (SDS-PAGE)
• form: lyophilized powder
• potency: 0.3-3.75 ng/mL EC₅₀
• quality: endotoxin tested
• mol wt: dimer 45 kDa (glycosylated)
• packaging: pkg of 10 μg
• manufacturer/tradename: HumanKine^®
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1 EU/μg
• UniProt accession no.: P15692
• storage temp.: −20°C
• Gene Information: human ... VEGFA(7422)

Beschreibung

General description

Recombinant human Vascular Endothelial Growth Factor 165 (VEGF165) is expressed in HEK 293 cells and supplied as a glycosylated homodimeric protein suitable for mammalian cell culture research. Glycosylation contributes to stability in cell growth media and other applications.

Application

VEGF165 has been used in endothelial cell culture research, including studies of cell proliferation, migration, and vascular‑related signaling in vitro.

Biochem/physiol Actions

Vascular Endothelial Growth Factor (VEGF165) is a mitogenic growth factor for endothelial cells and mediates cellular responses through VEGF receptor‑dependent signaling pathways in in vitro cell models. It is a potent angiogenic factor and mitogen that stimulates proliferation, migration and formation of endothelial cells.

Physical form

Lyophilized from a 0.2 μm filtered solution of 1X PBS

Analysis Note

The specific activity was determined by the dose-dependent production of luciferase in a Hek293T reporter cell line.

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable